Effect of dl-praeruptorin A on desmin and vimentin content in rat ischemia/reperfusion myocardiocytes.

Intermediate filament (IF) proteins have been thought to play a role in nuclear centration, organelle movement and maintenance of cell shape. dl-praeruptorin A (Pd-Ia), a novel Ca2+-influx blocker and K+-channel opener isolated from Chinese traditional herbal medicine Qian-Hu, has been demonstrated to inhibit expression of apoptosis related proteins and reduce the level of proinflammatory factors in ischemia/reperfusion myocardiocytes. Morphologically, whether Pd-Ia effects myocardiocyte IFs remains unclear. The purpose of this study was, for the first time, to evaluate the in vivo effects of Pd-Ia on IF desmin and vimentin content in order to further explore its cardioprotection against ischemia and elucidate its mechanism of action.
Rats underwent a 30 minutes coronary occlusion followed by 120 minutes reperfusion. Assessment of desmin and vimentin content of myocardiocytes was performed by immunohistochemistry, Western blot, Hematoxylin-Eosin staining and densitometry.
Pretreatment with i.v. infusion of Pd-Ia prior to ischemia significantly increased desmin and vimentin content and alleviated defects caused by the ischemia/reperfusion insult, e.g. with Pd-Ia at a dose of 0.5 or 1.0 mg/kg, integrated density values of desmin were increased from 61 478 +/- 10 074 to 177 408 +/- 10 395 and 195 784 +/- 20 057, and vimentin from 59 189 +/- 19 853 to 164 781 +/- 19 543 and 185 696 +/- 20 957 (P < 0.01, vs placebo), respectively. The recovery of desmin seemed to be stronger and appeared earlier than that of vimentin.
Pd-Ia protectively increased IF desmin and vimentin content in ischemia/reperfusion myocardiocytes, which might be partially responsible for its cardioprotection against ischemia.
AuthorsHe Chang, Xiao-yu Chu, Jun Zou, Tian-hui Chang
JournalChinese medical journal (Chin Med J (Engl)) Vol. 120 Issue 24 Pg. 2256-9 (Dec 20 2007) ISSN: 0366-6999 [Print] China
PMID18167213 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Coumarins
  • Desmin
  • Drugs, Chinese Herbal
  • Vimentin
  • praeruptorin A
  • Calcium
  • Animals
  • Blotting, Western
  • Calcium (metabolism)
  • Coumarins (pharmacology)
  • Desmin (analysis)
  • Dose-Response Relationship, Drug
  • Drugs, Chinese Herbal (pharmacology)
  • Female
  • Immunohistochemistry
  • Male
  • Myocardial Ischemia (drug therapy, metabolism)
  • Myocardial Reperfusion Injury (prevention & control)
  • Myocytes, Cardiac (chemistry, drug effects, pathology)
  • Rats
  • Rats, Wistar
  • Vimentin (analysis)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research network!

Choose Username:
Verify Password: