Abstract | PURPOSE:
Clusterin is a multifunctional glycoprotein. Its mRNA is ubiquitously expressed, with high levels in von Hippel-Lindau (VHL) target organs such as the brain, liver, kidney, and adrenal medulla. Decreased clusterin secretion has been reported in renal cell carcinoma associated with VHL disease. The purpose of this study was to investigate ocular clusterin expression in VHL disease. METHODS: This retrospective case series included nine eyes with retinal hemangioblastoma/ hemangioma associated with VHL disease, one eye from a patient with a history of VHL disease and central nervous system hemangioblastomas but without ocular lesions, one surgically-excised optic nerve with optic nerve hemangioblastoma/ hemangioma, and three normal control eyes. Ocular specimens were evaluated by routine histology, immunohistochemistry for clusterin expression, and molecular detection of clusterin transcripts within ocular VHL hemangioblastomas compared with normal tissue from the same eye using microdissection and quantitative real-time PCR. RESULTS: CONCLUSIONS:
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Authors | Min Zhou, Defen Shen, James E Head, Emily Y Chew, Patricia Chévez-Barrios, W Richard Green, Chi-Chao Chan |
Journal | Molecular vision
(Mol Vis)
Vol. 13
Pg. 2129-36
(Nov 15 2007)
ISSN: 1090-0535 [Electronic] United States |
PMID | 18079682
(Publication Type: Journal Article, Research Support, N.I.H., Intramural, Research Support, Non-U.S. Gov't)
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Chemical References |
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Topics |
- Adult
- Clusterin
(genetics, metabolism)
- Eye
(metabolism)
- Female
- Hemangioblastoma
(etiology, metabolism, pathology)
- Humans
- Immunohistochemistry
- Male
- Middle Aged
- Neuroglia
(metabolism)
- Optic Nerve Neoplasms
(etiology, metabolism, pathology)
- Polymerase Chain Reaction
- RNA, Messenger
(metabolism)
- Retina
(metabolism)
- Retinal Neoplasms
(etiology, metabolism, pathology)
- Retinal Vessels
(metabolism)
- Retrospective Studies
- von Hippel-Lindau Disease
(complications, metabolism)
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