Effective production and purification of the glycosylated TSOL18 antigen, which is protective against pig cysticercosis.

Abstract

Cysticercosis caused by Taenia solium metacestodes is a worldwide public health problem. Important progress in the development of effective and practical vaccines against this disease has been made. In this study, the promising T. solium oncospheral vaccine candidate named TSOL18 antigen was produced in a 5-liter fermentor. During the process of fermentation, the pH of the culture was always kept below 5.0, and in order to prevent foaming, an antifoam agent was added. In addition, the oxygen content of the culture was constantly kept at >50% in our experiment. A high level of the glycosylated protein (2.5 g/liter) was obtained, and the protein was easily purified by gel chromatography. Vaccination trials showed that the recombinant TSOL18 antigen induced 94 and 100% reductions in metacestode burdens in vaccinated pigs, obviously higher than the 89% reduction in pigs immunized with cysticercus crude extracts in trial 1. These are very promising results in the development of an efficient tool to control cysticercosis in Asia.

• Authors: Xuepeng Cai, Gailing Yuan, Yadong Zheng, Xuenong Luo, Shaohua Zhang, Juntao Ding, Zhizhong Jing, Chengping Lu
• Journal: Infection and immunity (Infect Immun) Vol. 76 Issue 2 Pg. 767-70 (Feb 2008) ISSN: 1098-5522 [Electronic] United States
• PMID: 18070907 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibodies, Helminth
• Antigens, Helminth
• Culture Media
• Glycoproteins
• Recombinant Proteins
• TSOL-18 antigen

Topics

• Animals
• Antibodies, Helminth (blood)
• Antigens, Helminth (biosynthesis, immunology, isolation & purification)
• Bioreactors
• Chromatography, Gel (methods)
• Cloning, Molecular
• Culture Media (chemistry)
• Cysticercosis (prevention & control)
• Glycoproteins (biosynthesis, immunology, isolation & purification)
• Humans
• Pichia (genetics)
• Recombinant Proteins (biosynthesis, immunology, isolation & purification)
• Swine
• Swine Diseases (prevention & control)
• Taenia solium (genetics, immunology, isolation & purification)