[Research of real-time fluorescent PCR in the rapid differential detection of H5, H9, H7 subtype avian influenza inactivated vaccines].

Abstract

Specific primers and TaqMan MGB probes were designed with Primer Express 2.0 software according to the conserved region of the H5, H9, H7 subtype AIV hemagglutinin gene to make research of real-time fluorescent one-step PCR in the differential detection of H5, H9, H7 subtype avian influenza inactivated vaccines. The result showed that the method was specific and reproducible. No cross-reaction was discovered with other avian disease vaccines. Real-time fluorescent PCR provided a specific, sensitive, rapid and convenient method for the subtype identification of avian influenza inactivated vaccines.

• Authors: Jian-Feng Han, Yi-Bao Ning, Li Song, Cheng-Huai Yang
• Journal: Sheng wu gong cheng xue bao = Chinese journal of biotechnology (Sheng Wu Gong Cheng Xue Bao) Vol. 23 Issue 5 Pg. 953-7 (Sep 2007) ISSN: 1000-3061 [Print] China
• PMID: 18051881 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Hemagglutinin Glycoproteins, Influenza Virus
• Influenza Vaccines
• Vaccines, Inactivated
• hemagglutinin, avian influenza A virus

Topics

• Animals
• Hemagglutinin Glycoproteins, Influenza Virus (immunology)
• Humans
• Influenza A Virus, H5N1 Subtype (immunology)
• Influenza A Virus, H7N7 Subtype (immunology)
• Influenza A Virus, H9N2 Subtype (immunology)
• Influenza A virus (classification, immunology)
• Influenza Vaccines (analysis, classification)
• Reverse Transcriptase Polymerase Chain Reaction (methods)
• Vaccines, Inactivated (analysis)