Antibody changes in experimental
anisakiasis were observed in 10 rabbits which were infected each with 10 Anisakis simplex larvae. The sera were collected before and on the 6th to the 95th day after the
infection. Using crude saline extract of Anisakis larvae as
antigen, specific
IgM and
IgG antibody levels were observed by ELISA and SDS-
polyacrylamide gel electrophoresis/immunoblot. Levels of specific-
IgM antibody were elevated from the 6th day, reached their peaks on the 11th day after the
infection, and dropped thereafter. Serum levels of
IgG antibody increased from the 6th day and reached their peak on the 26th day after the
infection, and decreased gradually thereafter. When SDS-PAGE of the
crude extract was done, at least forty-one SDS-
polypeptide bands were recognized. Of them,
IgM antibody reacted mainly to the bands of 168, 95, 74, 64, 51, 47 and 34 kDa while
IgG antibody reacted strongly to 168, 92, 85, 64, 58, 52, 42 and 40 kDa bands. The
crude extract showed negligible cross reactions with sera of other
parasitic diseases and normal control.