Abstract | BACKGROUND: Urinary excretion of desmosine has been reported to be increased in patients with pulmonary fibrosis; however, several investigators have pointed out that measuring urinary desmosine is not a very useful indicator of lung wall destruction. We developed a sensitive time resolved fluoroimmunoassay (TR-FIA) to identify trace amounts of desmosine in bronchoalveolar lavage fluid (BALF), and applied this method to analyse BALF samples from healthy subjects and patients with interstitial lung diseases. METHODS: RESULTS: The detectable limit of desmosine was 50 fmol/ml in the TR-FIA developed in this study. TR-FIA showed low cross-reactivity against amino acids. BALF desmosine levels were significantly higher in patients with idiopathic fibrosis and sarcoidosis compared with healthy subjects. CONCLUSIONS:
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Authors | Takaji Sato, Takashi Kajikuri, Yoshihiro Saito, Masahiko Chikuma, Sonoko Nagai |
Journal | Clinica chimica acta; international journal of clinical chemistry
(Clin Chim Acta)
Vol. 387
Issue 1-2
Pg. 113-9
(Jan 2008)
ISSN: 0009-8981 [Print] Netherlands |
PMID | 18028893
(Publication Type: Journal Article)
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Chemical References |
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Topics |
- Bronchoalveolar Lavage Fluid
(chemistry)
- Desmosine
(analysis)
- Fluorescent Antibody Technique
(methods)
- Humans
- Hydrolysis
- Reproducibility of Results
- Sensitivity and Specificity
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