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Protection against Shiga toxin-producing Escherichia coli infection by transcutaneous immunization with Shiga toxin subunit B.

Abstract
Enterohemorrhagic Escherichia coli (EHEC) strains are important human food-borne pathogens. EHEC strains elaborate potent Shiga toxins (Stx1, and/or Stx2) implicated in the development of hemorrhagic colitis (HC) or hemolytic-uremic syndrome (HUS). In this report, we evaluated the immunogenicity and protective efficacy of Stx1 subunit B (StxB1) administered by transcutaneous immunization (TCI). Three groups of Dutch Belted rabbits received patches containing StxB1, StxB1 in combination with Escherichia coli heat-labile enterotoxin (LT), or LT alone. An additional group of naïve rabbits served as controls. The protective efficacy following TCI with StxB1 was assessed by challenging rabbits with a virulent Stx1-producing strain, RDEC-H19A, capable of inducing HC and HUS in rabbits. Antibodies specific to StxB1 from serum and bile samples were determined by enzyme-linked immunosorbent assay and toxin neutralization test. Rabbits immunized with StxB1 demonstrated improved weight gain and reduced Stx-induced histopathology. Rabbits receiving StxB or StxB1/LT showed a significant increase in serum immunoglobulin G titers specific to StxB1 as well as toxin neutralization titers. These data demonstrated that the StxB delivered by TCI could induce significant systemic immune responses. Thus, Stx subunit B vaccine delivered by a patch for a high-risk population may be a practical approach to prevent (and/or reduce) Stx-induced pathology.
AuthorsC Zhu, J Yu, Z Yang, K Davis, H Rios, B Wang, G Glenn, E C Boedeker
JournalClinical and vaccine immunology : CVI (Clin Vaccine Immunol) Vol. 15 Issue 2 Pg. 359-66 (Feb 2008) ISSN: 1556-679X [Electronic] United States
PMID18003816 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, U.S. Gov't, Non-P.H.S.)
Chemical References
  • Adjuvants, Immunologic
  • Antibodies, Bacterial
  • Antitoxins
  • Bacterial Toxins
  • Enterotoxins
  • Escherichia coli Proteins
  • Protein Subunits
  • Vaccines, Subunit
  • Shiga Toxin
  • heat-labile enterotoxin, E coli
Topics
  • Adjuvants, Immunologic (administration & dosage)
  • Administration, Cutaneous
  • Animals
  • Antibodies, Bacterial (analysis, blood)
  • Antitoxins (analysis, blood)
  • Bacterial Toxins (administration & dosage)
  • Bile (immunology)
  • Body Weight (immunology)
  • Cecum (pathology)
  • Colitis (prevention & control)
  • Enterotoxins (administration & dosage)
  • Escherichia coli Infections (immunology, prevention & control)
  • Escherichia coli Proteins (administration & dosage)
  • Feces (microbiology)
  • Hemolytic-Uremic Syndrome (prevention & control)
  • Intestinal Mucosa (pathology)
  • Kidney (pathology)
  • Protein Subunits (administration & dosage, immunology)
  • Rabbits
  • Serum (immunology)
  • Shiga Toxin (administration & dosage, immunology)
  • Shiga-Toxigenic Escherichia coli (immunology)
  • Vaccines, Subunit (administration & dosage, immunology)

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