Abstract |
Enterohemorrhagic Escherichia coli (EHEC) strains are important human food-borne pathogens. EHEC strains elaborate potent Shiga toxins (Stx1, and/or Stx2) implicated in the development of hemorrhagic colitis (HC) or hemolytic-uremic syndrome (HUS). In this report, we evaluated the immunogenicity and protective efficacy of Stx1 subunit B (StxB1) administered by transcutaneous immunization (TCI). Three groups of Dutch Belted rabbits received patches containing StxB1, StxB1 in combination with Escherichia coli heat-labile enterotoxin (LT), or LT alone. An additional group of naïve rabbits served as controls. The protective efficacy following TCI with StxB1 was assessed by challenging rabbits with a virulent Stx1-producing strain, RDEC-H19A, capable of inducing HC and HUS in rabbits. Antibodies specific to StxB1 from serum and bile samples were determined by enzyme-linked immunosorbent assay and toxin neutralization test. Rabbits immunized with StxB1 demonstrated improved weight gain and reduced Stx-induced histopathology. Rabbits receiving StxB or StxB1/LT showed a significant increase in serum immunoglobulin G titers specific to StxB1 as well as toxin neutralization titers. These data demonstrated that the StxB delivered by TCI could induce significant systemic immune responses. Thus, Stx subunit B vaccine delivered by a patch for a high-risk population may be a practical approach to prevent (and/or reduce) Stx-induced pathology.
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Authors | C Zhu, J Yu, Z Yang, K Davis, H Rios, B Wang, G Glenn, E C Boedeker |
Journal | Clinical and vaccine immunology : CVI
(Clin Vaccine Immunol)
Vol. 15
Issue 2
Pg. 359-66
(Feb 2008)
ISSN: 1556-679X [Electronic] United States |
PMID | 18003816
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, U.S. Gov't, Non-P.H.S.)
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Chemical References |
- Adjuvants, Immunologic
- Antibodies, Bacterial
- Antitoxins
- Bacterial Toxins
- Enterotoxins
- Escherichia coli Proteins
- Protein Subunits
- Vaccines, Subunit
- Shiga Toxin
- heat-labile enterotoxin, E coli
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Topics |
- Adjuvants, Immunologic
(administration & dosage)
- Administration, Cutaneous
- Animals
- Antibodies, Bacterial
(analysis, blood)
- Antitoxins
(analysis, blood)
- Bacterial Toxins
(administration & dosage)
- Bile
(immunology)
- Body Weight
(immunology)
- Cecum
(pathology)
- Colitis
(prevention & control)
- Enterotoxins
(administration & dosage)
- Escherichia coli Infections
(immunology, prevention & control)
- Escherichia coli Proteins
(administration & dosage)
- Feces
(microbiology)
- Hemolytic-Uremic Syndrome
(prevention & control)
- Intestinal Mucosa
(pathology)
- Kidney
(pathology)
- Protein Subunits
(administration & dosage, immunology)
- Rabbits
- Serum
(immunology)
- Shiga Toxin
(administration & dosage, immunology)
- Shiga-Toxigenic Escherichia coli
(immunology)
- Vaccines, Subunit
(administration & dosage, immunology)
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