HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

GLP-1 amplifies insulin signaling by up-regulation of IRbeta, IRS-1 and Glut4 in 3T3-L1 adipocytes.

Abstract
Glucagon-like peptide-1 (7-36) amide (GLP-1) is an insulin secretagogue. Recently, many studies have shown GLP-1 can improve insulin resistance in peripheral tissues. In the present study, we investigated glucose uptake in 3T3-L1 adipocytes in either basal or insulin resistant state and dissected insulin signaling pathway in order to elucidate the molecular mechanisms of GLP-1 mediated improvement of insulin resistance. We found GLP-1 and its long lasting analogue, exendin 4 up-regulated basal IR, IRS-1 and Glut 4 expressions although they did not increase basal glucose uptake alone. However, GLP-1 and exendin-4 increased insulin mediated glucose uptake in intact and TNF-alpha treated 3T3-L1 adipocytes by up-regulation of phophorylated IRbeta, IRS-1, Akt and GSK-3beta. These results indicate that GLP-1 and its analogue exendin-4 can amplify insulin signaling in 3T3-L1 adipocytes by up-regulation of some crucial insulin signaling molecules.
AuthorsHong Gao, Xinjun Wang, Zhiguo Zhang, Yisheng Yang, Jun Yang, Xiaoying Li, Guang Ning
JournalEndocrine (Endocrine) Vol. 32 Issue 1 Pg. 90-5 (Aug 2007) ISSN: 1355-008X [Print] United States
PMID17992607 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Adaptor Proteins, Signal Transducing
  • Glucose Transporter Type 4
  • Insulin
  • Insulin Receptor Substrate Proteins
  • Irs1 protein, mouse
  • Peptides
  • Slc2a4 protein, mouse
  • Tumor Necrosis Factor-alpha
  • Venoms
  • Glucagon-Like Peptide 1
  • Exenatide
  • Receptor, Insulin
  • Glucose
Topics
  • 3T3-L1 Cells
  • Adaptor Proteins, Signal Transducing (metabolism)
  • Adipocytes (metabolism)
  • Animals
  • Exenatide
  • Glucagon-Like Peptide 1 (metabolism)
  • Glucose (metabolism)
  • Glucose Transporter Type 4 (metabolism)
  • Insulin (metabolism)
  • Insulin Receptor Substrate Proteins
  • Mice
  • Peptides (metabolism)
  • Receptor, Insulin (metabolism)
  • Signal Transduction (physiology)
  • Tumor Necrosis Factor-alpha
  • Up-Regulation
  • Venoms (metabolism)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: