Generation of a transgenic rice seed-based edible vaccine against house dust mite allergy.

Abstract

As an alternative approach to conventional allergen-specific immunotherapy, transgenic rice seed expressing a major house dust mite (HDM) allergen, Der p 1, was developed as an edible vaccine. The C-terminal KDEL-tagged Der p 1 allergen specifically accumulated in seed endosperm tissue under the control of the endosperm-specific GluB1 promoter. Der p 1 reached a maximum concentration of 58 microg/grain and was deposited in the endoplasmic reticulum (ER)-derived protein body I (PB-I). Plant-derived Der p 1 was posttranslationally modified with high-mannose-type glycan structures. Glycosylated Der p 1 displayed reduced IgE binding capacity in comparison with its unglycosylated counterpart in vitro. Our results indicate that transgenic Der p 1 rice seeds are a safe, potential oral delivery vaccine for the treatment of HDM allergy.

• Authors: Lijun Yang, Hiroyuki Kajiura, Kazuya Suzuki, Sakiko Hirose, Kazuhito Fujiyama, Fumio Takaiwa
• Journal: Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 365 Issue 2 Pg. 334-9 (Jan 11 2008) ISSN: 1090-2104 [Electronic] United States
• PMID: 17988639 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antigens, Dermatophagoides
• Arthropod Proteins
• Recombinant Proteins
• Vaccines, Edible
• Cysteine Endopeptidases
• Dermatophagoides pteronyssinus antigen p 1

Topics

• Animals
• Antigens, Dermatophagoides (genetics, immunology, metabolism, therapeutic use)
• Arthropod Proteins
• Cysteine Endopeptidases
• Oryza (genetics, metabolism)
• Plants, Genetically Modified (metabolism)
• Protein Engineering (methods)
• Pyroglyphidae (immunology)
• Recombinant Proteins (immunology, metabolism)
• Seeds (genetics, metabolism)
• Vaccines, Edible (immunology, metabolism, therapeutic use)