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Measurement of red blood cell antibodies in autoimmune hemolytic anemia.

Abstract
The enzyme-linked antiglobulin test (ELAT) was employed to measure the number of IgG molecules per red blood cell (IgG/RBC) in 11 patients with autoimmune hemolytic anemia (AIHA). All patients with AIHA had high levels of red cell-associated IgG (110-3, 650 IgG/RBC). The control group consisted of normal volunteers (N = 10) and patients with hereditary spherocytosis (N = 1), beta 0-thalassemia (N = 1), immunologic thrombocytopenic purpura (N = 3) and IgG multiple myeloma (N = 4). All control individuals presented low levels of red cell IgG (less than 38 IgG/RBC) with the exception of one of four patients with myeloma who had a mildly elevated value (50 IgG/RBC). Since the multiple myeloma patients had greater than 2 g/dl IgG, the possible nonspecific uptake of IgG onto the RBCs of patients with elevated serum IgG values did not interfere with the results of ELAT. ELAT proved to be a useful method for accurate quantification of the amount of IgG specifically bound on the surface of RBC of patients with AIHA.
AuthorsJ O Bordin, J C Souza-Pinto, J Kerbauy
JournalBrazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas (Braz J Med Biol Res) Vol. 24 Issue 9 Pg. 895-9 ( 1991) ISSN: 0100-879X [Print] Brazil
PMID1797282 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Immunoglobulin G
Topics
  • Adolescent
  • Adult
  • Aged
  • Anemia, Hemolytic, Autoimmune (immunology)
  • Coombs Test (methods)
  • Erythrocytes (immunology)
  • Female
  • Humans
  • Immunoglobulin G (analysis)
  • Male
  • Middle Aged

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