Abstract |
Two genes of wheat low-molecular-weight glutenin subunits (LMW-GS), B16 and P73, were cloned and expressed in E. coli. They were homologous to proteins encoded respectively at Glu-B3 and Glu-D3 loci. The N-terminal and C-terminal halves of B16 (NB16 and B16C) and the two chimeras combining the halves of the two genes (B16-P73 and P73- B16) were also expressed. All these constructs were compared for their reactivity with IgE from 24 patients suffering from different forms of wheat allergies. The results confirmed that LMW-GSs bound IgE in all adult allergies tested. Strong differences in reactivity between all the constructs were observed. They were disease-dependent. In wheat-dependent exercise-induced anaphylaxis ( WDEIA), the reactivity of the constructs depended partly on common epitopes with omega-5 gliadins but also on differences in molecule conformation. The presence of NB16 in the constructs greatly influenced their IgE reactivity.
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Authors | Jacques Snégaroff, Gérard Branlard, Isabelle Bouchez-Mahiout, Béatrice Laudet, Martina Tylichova, Thierry Chardot, Catherine Pecquet, Dominique Choudat, N Raison-Peyron, M Vigan, S Kerre, Michel Laurière |
Journal | Journal of agricultural and food chemistry
(J Agric Food Chem)
Vol. 55
Issue 24
Pg. 9837-45
(Nov 28 2007)
ISSN: 0021-8561 [Print] United States |
PMID | 17960887
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Plant Proteins
- Recombinant Proteins
- Immunoglobulin E
- Glutens
- glutenin
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Topics |
- Amino Acid Sequence
- Anaphylaxis
(immunology)
- Chimera
- Escherichia coli
- Exercise
- Glutens
(chemistry, genetics, immunology)
- Humans
- Immunoglobulin E
(immunology)
- Molecular Sequence Data
- Molecular Weight
- Plant Proteins
(chemistry, genetics, immunology)
- Recombinant Proteins
(chemistry, genetics, immunology)
- Wheat Hypersensitivity
(diagnosis, immunology)
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