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Recombinant proteins and peptides as tools for studying IgE reactivity with low-molecular-weight glutenin subunits in some wheat allergies.

Abstract
Two genes of wheat low-molecular-weight glutenin subunits (LMW-GS), B16 and P73, were cloned and expressed in E. coli. They were homologous to proteins encoded respectively at Glu-B3 and Glu-D3 loci. The N-terminal and C-terminal halves of B16 (NB16 and B16C) and the two chimeras combining the halves of the two genes (B16-P73 and P73- B16) were also expressed. All these constructs were compared for their reactivity with IgE from 24 patients suffering from different forms of wheat allergies. The results confirmed that LMW-GSs bound IgE in all adult allergies tested. Strong differences in reactivity between all the constructs were observed. They were disease-dependent. In wheat-dependent exercise-induced anaphylaxis (WDEIA), the reactivity of the constructs depended partly on common epitopes with omega-5 gliadins but also on differences in molecule conformation. The presence of NB16 in the constructs greatly influenced their IgE reactivity.
AuthorsJacques Snégaroff, Gérard Branlard, Isabelle Bouchez-Mahiout, Béatrice Laudet, Martina Tylichova, Thierry Chardot, Catherine Pecquet, Dominique Choudat, N Raison-Peyron, M Vigan, S Kerre, Michel Laurière
JournalJournal of agricultural and food chemistry (J Agric Food Chem) Vol. 55 Issue 24 Pg. 9837-45 (Nov 28 2007) ISSN: 0021-8561 [Print] United States
PMID17960887 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Plant Proteins
  • Recombinant Proteins
  • Immunoglobulin E
  • Glutens
  • glutenin
Topics
  • Amino Acid Sequence
  • Anaphylaxis (immunology)
  • Chimera
  • Escherichia coli
  • Exercise
  • Glutens (chemistry, genetics, immunology)
  • Humans
  • Immunoglobulin E (immunology)
  • Molecular Sequence Data
  • Molecular Weight
  • Plant Proteins (chemistry, genetics, immunology)
  • Recombinant Proteins (chemistry, genetics, immunology)
  • Wheat Hypersensitivity (diagnosis, immunology)

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