Abstract |
Abnormal lipid metabolism has been implicated in the pathogenesis of many neural system diseases, including epilepsy. Pentylenetetrazol (PTZ)-induced kindling in rodents is considered a model of human absence epilepsy and myoclonic, generalized tonic-clonic seizure. In an effort to further understand the mechanism for PTZ-induced seizure, we analyzed crude lipids and sphingolipids in the cortex, hippocampus, and brain stem of normal and PTZ-rats using delayed extraction matrix-assisted laser desorption ionization time-of-flight mass spectrometry (DE MALDI-TOF-MS). It was found that phosphatidylcholines dominated the crude lipids in different tissues and there were no obvious differences in crude lipid profiles of different tissues between normal and PTZ-rats. However, ceramide, sphingomyelins, and ceramide-monohexoside were differently distributed in normal and PTZ-rats. Using the reference mass spectra method established in our laboratory, it was shown that sphingomyelins and ceramide-monohexoside levels were elevated in the brain tissues of PTZ-rats. Ceramide levels were found to be higher in brain stem than in cortex and hippocampus of normal rats, and PTZ caused a general decrease in ceramide levels. These data suggest that changes in sphingolipid metabolism contribute to PTZ-induced seizure.
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Authors | Xiaoqiong Ma, Guangyi Liu, Shuang Wang, Zhong Chen, Maode Lai, Ziyang Liu, Jun Yang |
Journal | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
(J Chromatogr B Analyt Technol Biomed Life Sci)
Vol. 859
Issue 2
Pg. 170-7
(Nov 15 2007)
ISSN: 1570-0232 [Print] Netherlands |
PMID | 17931985
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Sphingolipids
- Pentylenetetrazole
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Topics |
- Animals
- Brain
(metabolism)
- Brain Chemistry
- Kindling, Neurologic
(drug effects, metabolism)
- Male
- Pentylenetetrazole
- Rats
- Rats, Sprague-Dawley
- Seizures
(chemically induced, metabolism)
- Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
- Sphingolipids
(metabolism)
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