Tocopherols are present in significant amounts in
vegetable oils used in human foods. The most prevalent
tocopherols in foods are the alpha, beta, gamma, and delta variants with (RRR) stereochemistry.
Tocopherols are lipophilic phenolic
antioxidants, produced by plants. In the United States,
gamma-tocopherol is the most prominent dietary
tocopherol due to its high amount in the dominant commercially produced
vegetable oils such as soybean, corn, and
cottonseed. In this report, experiments were designed to study the inhibitory effect of mixed
tocopherols against
N-methyl-N-nitrosourea-induced mammary
tumor growth in female Sprague-Dawley rats. Beginning at 21 days of age, rats were treated with a single
intraperitoneal injection of 50 mg/kg
body weight of
N-methyl-N-nitrosourea. One wk later, the rats were fed experimental diets containing 0 or 0.1% mixed
tocopherols containing over 50%
gamma-tocopherol. At 9 wk after
N-methyl-N-nitrosourea treatment, all rats were evaluated for inhibition of mammary
tumor growth and
proliferating cell nuclear antigen. Dietary administration of mixed
tocopherols significantly suppressed mammary
tumor growth (P < 0.05) and
proliferating cell nuclear antigen (P < 0.01) and also moderately suppressed
tumor multiplicity. The treatment increased the serum levels of gamma- and delta-
tocopherols without affecting the
body weight. The results of this study suggest that mixed
tocopherols may be safe and effective agents for the prevention of
breast cancer.