Alpha-lipoic acid modulates gut inflammation induced by trinitrobenzene sulfonic acid in rats.

Abstract

BACKGROUND AND AIM:

Alpha-lipoic acid (ALA) has been shown to combat oxidative stress by quenching a variety of reactive oxygen species. It is involved in the regeneration of exogenous and endogenous antioxidants, chelation of metal ions, and repair of oxidized proteins. This study aimed to evaluate the potential beneficial effect of ALA on trinitrobenzenesulfonic acid (TNBS)-induced gut ileitis and colitis in rats.

METHOD:

After 48 h of fasting, Sprague-Dawley rats underwent a laparotomy under ether anesthesia. TNBS solution 30 mg/mL in 40% ethanol (1 mL) was injected into the lumen, 10 cm proximal to the ileocolonic junction to induce ileitis or intrarectally 8 cm proximal to the anal sphincter to induce colitis. ALA (25 mg/kg intraperitoneally, twice a day) was given after induction of inflammation and continued for 3 days. All animals were decapitated 3 days after induction of the inflammation. The mucosal lesions of the ileum and colon were scored macroscopically and microscopically. Samples were taken for the measurement of malondialdehyde (MDA) and glutathione (GSH) levels, tissue-associated myeloperoxidase (MPO) activity and luminol- or lucigenin-enhanced chemiluminescence (CL).

RESULTS:

Macroscopic scores, morphological changes and increased tissue lipid peroxidation with a concomitant reduction in GSH of the ileitis or colitis groups were all reversed by treatment with ALA. ALA treatment was also effective in improving tissue MPO activity and CL values, which were elevated in untreated ileitis or colitis groups.

CONCLUSION:

ALA is beneficial in TNBS-induced gut inflammation in rats via suppression of neutrophil accumulation, preservation of endogenous glutathione and inhibition of reactive oxidant generation.

• Authors: Meltem Kolgazi, Nermina Jahovic, Meral Yüksel, Feriha Ercan, Inci Alican
• Journal: Journal of gastroenterology and hepatology (J Gastroenterol Hepatol) Vol. 22 Issue 11 Pg. 1859-65 (Nov 2007) ISSN: 0815-9319 [Print] Australia
• PMID: 17914960 (Publication Type: Journal Article)

Chemical References

• Anti-Inflammatory Agents
• Antioxidants
• Gastrointestinal Agents
• Reactive Oxygen Species
• Malondialdehyde
• Thioctic Acid
• Trinitrobenzenesulfonic Acid
• Peroxidase
• Glutathione

Topics

• Animals
• Anti-Inflammatory Agents (pharmacology, therapeutic use)
• Antioxidants (pharmacology, therapeutic use)
• Colitis (chemically induced, drug therapy, metabolism, pathology)
• Colon (drug effects, metabolism, pathology)
• Disease Models, Animal
• Gastrointestinal Agents (pharmacology, therapeutic use)
• Glutathione (metabolism)
• Ileitis (chemically induced, drug therapy, metabolism, pathology)
• Ileum (drug effects, metabolism, pathology)
• Lipid Peroxidation (drug effects)
• Malondialdehyde (metabolism)
• Neutrophil Infiltration (drug effects)
• Neutrophils (drug effects, enzymology)
• Oxidative Stress (drug effects)
• Peroxidase (metabolism)
• Rats
• Rats, Sprague-Dawley
• Reactive Oxygen Species (metabolism)
• Thioctic Acid (pharmacology, therapeutic use)
• Trinitrobenzenesulfonic Acid