Abstract |
The CC chemokine receptor 7 (CCR7) and its two ligands, CCL21 and CCL19, play an important role in migration of immune cells to lymphoid tissue. To analyze the function of CCR7 in T cell immunity to infectious agents in vivo, transgenic (tg) mice expressing CCL21 in an ubiquitous fashion were generated. These mice contained high amounts of CCL21 in the serum ( approximately 0.3 microg/ml that resulted in CCR7 down-regulation and in a strongly impaired migration of T cells toward CCL21 in vitro. Lymph nodes in CCL21-tg mice were reduced in size but with intact microanatomy and normal distribution of T and B cells. CCL21-tg mice showed a significantly decreased CD8 T cell response to lymphocytic choriomeningitis virus after footpad infection, whereas the response after systemic infection was not altered. Likewise, the CD4 T cell response to footpad infection with Leishmania major was considerably lowered and CCL21-tg mice failed to clear parasites from infected skin. Taken together, these data demonstrate the importance of CCR7 in mediating T cell immunity to viral and parasitic pathogens after local infection.
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Authors | Heike Unsoeld, Katja Mueller, Ulrike Schleicher, Christian Bogdan, Jörg Zwirner, David Voehringer, Hanspeter Pircher |
Journal | International immunology
(Int Immunol)
Vol. 19
Issue 11
Pg. 1281-9
(Nov 2007)
ISSN: 0953-8178 [Print] England |
PMID | 17914120
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Chemokine CCL21
- Receptors, CCR7
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Topics |
- Animals
- Arenaviridae Infections
(immunology)
- CD4-Positive T-Lymphocytes
(immunology, metabolism)
- CD8-Positive T-Lymphocytes
(immunology, metabolism)
- Chemokine CCL21
(blood, genetics, immunology)
- Leishmania major
(immunology)
- Leishmaniasis, Cutaneous
(immunology)
- Lymph Nodes
(cytology, immunology, metabolism)
- Lymphocytic choriomeningitis virus
(immunology)
- Mice
- Mice, Inbred C57BL
- Mice, Transgenic
- Receptors, CCR7
(immunology, metabolism)
- T-Lymphocytes
(immunology)
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