Abstract | OBJECTIVE: DESIGN: Laboratory study. SETTING: University hospital laboratory. SUBJECTS: Anesthetized male Wistar rats. INTERVENTIONS: To test the hypothesis, rats received saline or lipopolysaccharide (1 mg/kg). At 2, 4, 8, 16, or 24 hrs later, blood samples and lung tissue were taken to determine messenger RNA, protein concentration, and activity of inducible nitric oxide synthase and heme oxygenase-1. In additional experiments, rats were challenged with lipopolysaccharide (1 mg/kg) and subjected to Gram-negative ( lipopolysaccharide) or Gram-positive ( lipoteichoic acid and peptidoglycan) shock 24 hrs later. These studies were carried out in the presence and absence of inducible nitric oxide synthase or heme oxygenase-1 inhibitors (1400W or tin protoporphyrin IX). Following 6 hrs of shock, lung tissue was taken to determine lung damage and heme oxygenase-1 concentration and activity. MEASUREMENTS AND MAIN RESULTS: CONCLUSIONS:
|
Authors | Alexander Koch, Olaf Boehm, Paula A Zacharowski, Stephan A Loer, Jörg Weimann, Hauke Rensing, Simon J Foster, Rene Schmidt, Reinhard Berkels, Sonja Reingruber, Kai Zacharowski |
Journal | Critical care medicine
(Crit Care Med)
Vol. 35
Issue 12
Pg. 2775-84
(Dec 2007)
ISSN: 0090-3493 [Print] United States |
PMID | 17901834
(Publication Type: Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't)
|
Chemical References |
- Lipopolysaccharides
- Nitric Oxide Synthase Type II
- Heme Oxygenase-1
|
Topics |
- Animals
- Desensitization, Immunologic
- Gene Expression Regulation
(immunology)
- Gram-Negative Bacterial Infections
(immunology)
- Gram-Positive Bacterial Infections
(immunology)
- Heme Oxygenase-1
(genetics, immunology, metabolism)
- Immune Tolerance
(immunology)
- Lipopolysaccharides
(immunology)
- Lung
(metabolism, pathology)
- Male
- Nitric Oxide Synthase Type II
(immunology, metabolism)
- Rats
- Rats, Wistar
- Shock, Septic
(immunology, pathology)
|