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KEG1/YFR042w encodes a novel Kre6-binding endoplasmic reticulum membrane protein responsible for beta-1,6-glucan synthesis in Saccharomyces cerevisiae.

Abstract
KEG1/YFR042w of Saccharomyces cerevisiae is an essential gene that encodes a 200-amino acid polypeptide with four predicted transmembrane domains. The green fluorescent protein- or Myc(6)-tagged Keg1 protein showed the typical characteristics of an integral membrane protein and was found in the endoplasmic reticulum by fluorescence imaging. Immunoprecipitation from the Triton X-100-solubilized cell lysate revealed that Keg1 binds to Kre6, which has been known to participate in beta-1,6-glucan synthesis. To analyze the essential function of Keg1 in more detail, we constructed temperature-sensitive mutant alleles by error-prone polymerase chain reaction. The keg1-1 mutant cells showed a common phenotype with Deltakre6 mutant including hypersensitivity to Calcofluor white, reduced sensitivity to the K1 killer toxin, and reduced content of beta-1,6-glucan in the cell wall. These results suggest that Keg1 and Kre6 have a cooperative role in beta-1,6-glucan synthesis in S. cerevisiae.
AuthorsKosuke Nakamata, Tomokazu Kurita, M Shah Alam Bhuiyan, Keisuke Sato, Yoichi Noda, Koji Yoda
JournalThe Journal of biological chemistry (J Biol Chem) Vol. 282 Issue 47 Pg. 34315-24 (Nov 23 2007) ISSN: 0021-9258 [Print] United States
PMID17893149 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Benzenesulfonates
  • Fluorescent Dyes
  • K1 killer toxin
  • KEG1 protein, S cerevisiae
  • KRE6 protein, S cerevisiae
  • Killer Factors, Yeast
  • Membrane Proteins
  • Mycotoxins
  • Saccharomyces cerevisiae Proteins
  • beta-Glucans
  • beta-1,6-glucan
  • C.I. Fluorescent Brightening Agent 28
Topics
  • Benzenesulfonates (pharmacology)
  • Cell Wall (genetics, metabolism)
  • Drug Resistance, Fungal (drug effects, genetics)
  • Endoplasmic Reticulum (genetics, metabolism)
  • Fluorescent Dyes (pharmacology)
  • Killer Factors, Yeast
  • Membrane Proteins (genetics, metabolism)
  • Mutation
  • Mycotoxins (pharmacology)
  • Protein Binding (drug effects, physiology)
  • Saccharomyces cerevisiae (genetics, metabolism)
  • Saccharomyces cerevisiae Proteins (genetics, metabolism)
  • beta-Glucans (metabolism)

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