KEG1/YFR042w encodes a novel Kre6-binding endoplasmic reticulum membrane protein responsible for beta-1,6-glucan synthesis in Saccharomyces cerevisiae.

KEG1/YFR042w of Saccharomyces cerevisiae is an essential gene that encodes a 200-amino acid polypeptide with four predicted transmembrane domains. The green fluorescent protein- or Myc(6)-tagged Keg1 protein showed the typical characteristics of an integral membrane protein and was found in the endoplasmic reticulum by fluorescence imaging. Immunoprecipitation from the Triton X-100-solubilized cell lysate revealed that Keg1 binds to Kre6, which has been known to participate in beta-1,6-glucan synthesis. To analyze the essential function of Keg1 in more detail, we constructed temperature-sensitive mutant alleles by error-prone polymerase chain reaction. The keg1-1 mutant cells showed a common phenotype with Deltakre6 mutant including hypersensitivity to Calcofluor white, reduced sensitivity to the K1 killer toxin, and reduced content of beta-1,6-glucan in the cell wall. These results suggest that Keg1 and Kre6 have a cooperative role in beta-1,6-glucan synthesis in S. cerevisiae.
AuthorsKosuke Nakamata, Tomokazu Kurita, M Shah Alam Bhuiyan, Keisuke Sato, Yoichi Noda, Koji Yoda
JournalThe Journal of biological chemistry (J Biol Chem) Vol. 282 Issue 47 Pg. 34315-24 (Nov 23 2007) ISSN: 0021-9258 [Print] United States
PMID17893149 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Benzenesulfonates
  • Fluorescent Dyes
  • K1 killer toxin
  • KEG1 protein, S cerevisiae
  • KRE6 protein, S cerevisiae
  • Killer Factors, Yeast
  • Membrane Proteins
  • Mycotoxins
  • Saccharomyces cerevisiae Proteins
  • beta-Glucans
  • beta-1,6-glucan
  • C.I. Fluorescent Brightening Agent 28
  • Benzenesulfonates (pharmacology)
  • Cell Wall (genetics, metabolism)
  • Drug Resistance, Fungal (drug effects, genetics)
  • Endoplasmic Reticulum (genetics, metabolism)
  • Fluorescent Dyes (pharmacology)
  • Killer Factors, Yeast
  • Membrane Proteins (genetics, metabolism)
  • Mutation
  • Mycotoxins (pharmacology)
  • Protein Binding (drug effects, physiology)
  • Saccharomyces cerevisiae (genetics, metabolism)
  • Saccharomyces cerevisiae Proteins (genetics, metabolism)
  • beta-Glucans (metabolism)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research network!

Choose Username:
Verify Password: