[Cerulenin changes apoptosis related genes expression in multiple myeloma cell line U266].

Abstract

OBJECTIVE:

To study the expression changes of apoptosis related genes induced by cerulenin in multiple myeloma cell line U266 and explore its molecular mechanism.

METHODS:

The expression changes of 96 apoptosis related genes were analyzed by superArray cDNA in U266 cells treated with cerulenin (20 microg/ml) for 12 h. Semi-quantitative RT-PCR was used to confirm the representative expression changes genes, Rip2, caspase 9 and TRAF2.

RESULTS:

After treated with cerulenin for 12 h, 44 apoptosis related genes expression in the U266 cells were changed, among which 41 were over 2 fold increase and 3 over 2 fold decrease. The expression of caspase 9 was increased markedly, indicating that mitochondria pathway played a key role in cerulenin inducing apoptosis and TRAF2 expression change suggested that nuclear factor (NF) participates in cerulenin inducing apoptosis.

CONCLUSION:

The death acceptor signaling pathway and the death acceptor non-dependence signaling pathway co-regulate cerulenin inducing apoptosis in U266 cells. Mitochondria pathway played the key role and nuclear factor (NF) participates in the apoptosis process.

• Authors: Wei-Qin Wang, Xiao-Ying Zhao, Xu-Bo Gong, Xiao-Hong Zhang
• Journal: Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi (Zhonghua Xue Ye Xue Za Zhi) Vol. 28 Issue 4 Pg. 239-42 (Apr 2007) ISSN: 0253-2727 [Print] China
• PMID: 17877200 (Publication Type: English Abstract, Journal Article)

Chemical References

• Cerulenin

Topics

• Apoptosis (drug effects, genetics)
• Cell Line, Tumor
• Cerulenin (pharmacology)
• Gene Expression Profiling
• Gene Expression Regulation, Neoplastic
• Humans
• Multiple Myeloma (genetics, metabolism, pathology)
• Signal Transduction (drug effects)