Abstract |
The membrane (M) protein of severe acute respiratory syndrome coronavirus (SARS-CoV) is a major glycoprotein with multiple biological functions. In this study, we found that memory T cells against M protein were persistent in recovered SARS patients by detecting gamma interferon (IFN-gamma) production using ELISA and ELISpot assays. Flow cytometric analysis showed that both CD4+ and CD8+ T cells were involved in cellular responses to SARS-CoV M antigen. Furthermore, memory CD8+ T cells displayed an effector memory cell phenotype expressing CD45RO- CCR7- CD62L-. In contrast, the majority of IFN-gamma+ CD4+ T cells were central memory cells with the expression of CD45RO+ CCR7+ CD62L-. The epitope screening from 30 synthetic overlapping peptides that cover the entire SARS-CoV M protein identified four human T-cell immunodominant peptides, p21-44, p65-91, p117-140 and p200-220. All four immunodominant peptides could elicit cellular immunity with a predominance of CD8+ T-cell response. This data may have important implication for developing SARS vaccines.
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Authors | Litao Yang, Hui Peng, Zhaoling Zhu, Gang Li, Zitong Huang, Zhixin Zhao, Richard A Koup, Robert T Bailer, Changyou Wu |
Journal | The Journal of general virology
(J Gen Virol)
Vol. 88
Issue Pt 10
Pg. 2740-2748
(Oct 2007)
ISSN: 0022-1317 [Print] England |
PMID | 17872527
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antigens, Viral
- Viral Matrix Proteins
- Interferon-gamma
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Topics |
- Adult
- Antigens, Viral
(immunology)
- CD4-Positive T-Lymphocytes
(immunology)
- CD8-Positive T-Lymphocytes
(immunology)
- Enzyme-Linked Immunosorbent Assay
- Female
- Humans
- Immunologic Memory
- Interferon-gamma
(immunology)
- Male
- Reference Values
- Severe acute respiratory syndrome-related coronavirus
(immunology)
- Severe Acute Respiratory Syndrome
(immunology)
- Viral Matrix Proteins
(immunology)
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