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Persistent memory CD4+ and CD8+ T-cell responses in recovered severe acute respiratory syndrome (SARS) patients to SARS coronavirus M antigen.

Abstract
The membrane (M) protein of severe acute respiratory syndrome coronavirus (SARS-CoV) is a major glycoprotein with multiple biological functions. In this study, we found that memory T cells against M protein were persistent in recovered SARS patients by detecting gamma interferon (IFN-gamma) production using ELISA and ELISpot assays. Flow cytometric analysis showed that both CD4+ and CD8+ T cells were involved in cellular responses to SARS-CoV M antigen. Furthermore, memory CD8+ T cells displayed an effector memory cell phenotype expressing CD45RO- CCR7- CD62L-. In contrast, the majority of IFN-gamma+ CD4+ T cells were central memory cells with the expression of CD45RO+ CCR7+ CD62L-. The epitope screening from 30 synthetic overlapping peptides that cover the entire SARS-CoV M protein identified four human T-cell immunodominant peptides, p21-44, p65-91, p117-140 and p200-220. All four immunodominant peptides could elicit cellular immunity with a predominance of CD8+ T-cell response. This data may have important implication for developing SARS vaccines.
AuthorsLitao Yang, Hui Peng, Zhaoling Zhu, Gang Li, Zitong Huang, Zhixin Zhao, Richard A Koup, Robert T Bailer, Changyou Wu
JournalThe Journal of general virology (J Gen Virol) Vol. 88 Issue Pt 10 Pg. 2740-2748 (Oct 2007) ISSN: 0022-1317 [Print] England
PMID17872527 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Antigens, Viral
  • Viral Matrix Proteins
  • Interferon-gamma
Topics
  • Adult
  • Antigens, Viral (immunology)
  • CD4-Positive T-Lymphocytes (immunology)
  • CD8-Positive T-Lymphocytes (immunology)
  • Enzyme-Linked Immunosorbent Assay
  • Female
  • Humans
  • Immunologic Memory
  • Interferon-gamma (immunology)
  • Male
  • Reference Values
  • Severe acute respiratory syndrome-related coronavirus (immunology)
  • Severe Acute Respiratory Syndrome (immunology)
  • Viral Matrix Proteins (immunology)

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