Abstract | OBJECTIVE: To set up animal models of the lung cancer induced by Yunnan tin mineral dusts (no radon) in F344 rats and to explore the process of carcinogenesis and pathologic alterations in various stages of malignant transformation in the animal models. METHODS: One hundred and ninety F344 rats were randomly divided into Yunnan tin mineral dust group (100 rats), furfural physiological saline group (30 rats), physiological saline group (30 rats) and normal control group (30 rats). The intratracheal instillation with mass fraction of 6% suspension liquid mixture Yunnan tin mineral dusts, volume fraction of 2% furfural physiological saline and physiological saline 0.2 ml was performed in the rates once per week respectively except normal control group. Then the rats were sacrificed in batch periodically after one week. The last rat was exposed to the tin mine dusts for 100 weeks. The morphological process and tumor formation were dynamically observed under LM and TEM. Immunohistochemistry detection of cytokeratin of High MW and low MW was used for tumor classification. Pollak stein was used to evaluate the development of fibrosis of lung in the rats. RESULTS: CONCLUSION:
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Authors | Yong-hua Ruan, Hai-rong Hua, Qian Gao, Jing-ling Song, Rui Liang, Ke-wei Jin |
Journal | Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases
(Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi)
Vol. 25
Issue 6
Pg. 331-5
(Jun 2007)
ISSN: 1001-9391 [Print] China |
PMID | 17723188
(Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
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Topics |
- Animals
- Disease Models, Animal
- Dust
- Female
- Lung
(drug effects, pathology)
- Lung Neoplasms
(chemically induced, pathology)
- Male
- Rats
- Rats, Inbred F344
- Tin
(adverse effects)
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