Antioxidant activities of Toona Sinensis leaves extracts using different antioxidant models.

Abstract

The aim of the present study was to investigate the antioxidant activity of aqueous extracts of Toona sinensis (TS; 0-100 microg/mL) and gallic acid (0-50 microg/mL), with the purified natural phenolic components evaluated using different antioxidant models. It was found that the TS extracts and gallic acid possess effective antioxidant activity against various oxidative systems in vitro, including the scavenging of free and superoxide anion radicals, reducing power, and metal chelation. However, antioxidant activity in terms of metal chelation was not observed for the gallic acid. Moreover, TS extracts and gallic acid appear to possess powerful antioxidant properties with respect to oxidative modification of human LDL induced by CuSO4, AAPH or sodium nitroprusside, as assessed by the relative electrophoretic mobility, TBARS formation, and cholesterol degradation of oxidized LDL. Furthermore, AAPH-induced oxidative hemolysis, lipid peroxidation, and decline in superoxide dismutase (SOD) activity in human erythrocytes were prevented by both the TS extracts and the gallic acid. Our findings suggest that T. sinensis may act as a chemopreventative agent, providing antioxidant properties and offering effective protection from atherogenesis.

• Authors: You-Cheng Hseu, Wen-Huei Chang, Chee-Shan Chen, Jiunn-Wang Liao, Chin-Jung Huang, Fung-Jou Lu, Yi-Chen Chia, Hseng-Kuang Hsu, Jia-Jiuan Wu, Hsin-Ling Yang
• Journal: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association (Food Chem Toxicol) Vol. 46 Issue 1 Pg. 105-14 (Jan 2008) ISSN: 0278-6915 [Print] England
• PMID: 17703862 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antioxidants
• Biphenyl Compounds
• Chelating Agents
• Cholesterol, LDL
• Free Radical Scavengers
• Picrates
• Plant Extracts
• Thiobarbituric Acid Reactive Substances
• Superoxides
• Nitroprusside
• Gallic Acid
• 1,1-diphenyl-2-picrylhydrazyl
• Copper Sulfate

Topics

• Animals
• Antioxidants (chemistry, pharmacology)
• Biphenyl Compounds
• Chelating Agents (chemistry)
• Cholesterol, LDL (chemistry)
• Copper Sulfate (chemistry)
• Electrophoretic Mobility Shift Assay
• Erythrocytes (drug effects)
• Free Radical Scavengers (chemistry)
• Gallic Acid (pharmacology)
• Hemolysis (drug effects)
• In Vitro Techniques
• Lipid Peroxidation (drug effects)
• Meliaceae (chemistry)
• Nitroprusside (chemistry)
• Oxidation-Reduction
• Picrates (chemistry)
• Plant Extracts (chemistry, pharmacology)
• Plant Leaves (chemistry)
• Superoxides (chemistry)
• Thiobarbituric Acid Reactive Substances (chemistry)