Abstract | OBJECTIVE: METHODS: RESULTS: Expressions of the ERRalpha were significantly increased in the endometrial cancer cells transfected with pSG- ERRalpha plasmid; expression of the ERRalpha mRNA in HEC-1A cell was 9644.4 copies/ng, HEC-1B: 9835.3 copies/ng, and Ishikawa: 8008.6 copies/ng (P < 0.01); expression of the ERRalpha protein in HEC-1A cell was 1.128, HEC-1B: 1.104, and Ishikawa: 1.008 (P < 0.05). Flow cytometry showed over-expression of ERRalpha was accompanied by increased HEC-1A and HEC-1B cells in S and G(2)/M phase (P < 0.01), while this could not be observed in the estrogen receptor (ER) positive endometrial cancer cell line Ishikawa. Furthermore, cellular growth analysis showed that over-expression of ERRalpha induced cell growth increase of the ER negative endometrial cancer cells HEC-1A and HEC-1B (P < 0.05). CONCLUSION:
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Authors | Peng-Ming Sun, Li-Hui Wei, Min Gao, Jian-Liu Wang, Li-Jun Zhao, Da-Peng Wang, Jun-Xiao Zhang |
Journal | Zhonghua fu chan ke za zhi
(Zhonghua Fu Chan Ke Za Zhi)
Vol. 42
Issue 6
Pg. 408-11
(Jun 2007)
ISSN: 0529-567X [Print] China |
PMID | 17697604
(Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- ERRalpha estrogen-related receptor
- RNA, Messenger
- Receptors, Estrogen
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Topics |
- Blotting, Western
- Cell Cycle
- Cell Line, Tumor
- Cell Proliferation
- Endometrial Neoplasms
(genetics, metabolism, pathology)
- Female
- Flow Cytometry
- Gene Expression Regulation, Neoplastic
- Humans
- Plasmids
- RNA, Messenger
(biosynthesis, genetics)
- Receptors, Estrogen
(biosynthesis, genetics, physiology)
- Reverse Transcriptase Polymerase Chain Reaction
- Transfection
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