Abstract | BACKGROUND: METHODS: We quantified IFNAR mRNA expression in paired tumor and non- tumor samples from the surgical specimens of 103 consecutive patients with RCC using a real-time reverse transcription polymerase chain reaction (RT-PCR), and IFNAR2 protein using Western blotting. RESULTS: The absolute level of IFNAR1 and IFNAR2 mRNAs in tumor and non- tumor tissues did not correlate with the malignant and metastatic profiles. The relative yields of the PCR product from the tumor tissue to that from the corresponding non- tumor tissue (T/N) for the expression of IFNAR mRNAs were calculated. While the T/N ratio of IFNAR1 did not correlate with any factor, a high T/N ratio of IFNAR2 correlated with poor differentiation (P < 0.05), local invasion (P < 0.001), and metastasis (P < 0.0001). By multivariate analysis, a high T/N ratio of IFNAR2 predicted a shortened overall survival in all cases (P < 0.05) and a shorter disease-free survival in those without metastasis (M0; 68 cases, P < 0.05). Impressively, patients with a poorer response to IFN-alpha treatment had a higher IFNAR2 T/N ratio than those who had a good response (P < 0.05). IFNAR2c protein expression was higher in the primary tumors in patients with metastases (M1; 35 cases) compared to those without ( P < 0.0001). CONCLUSION: IFNAR2 is associated with the progression of RCC.
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Authors | Takao Kamai, Yoshiaki Yanai, Kyoko Arai, Hideyuki Abe, Tomonori Yamanishi, Masashi Kurimoto, Ken-Ichiro Yoshida |
Journal | BMC cancer
(BMC Cancer)
Vol. 7
Pg. 159
(Aug 15 2007)
ISSN: 1471-2407 [Electronic] England |
PMID | 17697365
(Publication Type: Journal Article)
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Chemical References |
- IFNAR1 protein, human
- IFNAR2 protein, human
- Interferon-alpha
- RNA, Messenger
- Receptor, Interferon alpha-beta
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Topics |
- Adult
- Aged
- Aged, 80 and over
- Blotting, Western
- Carcinoma, Renal Cell
(genetics, metabolism, secondary)
- Female
- Humans
- Interferon-alpha
(pharmacology)
- Kidney Neoplasms
(genetics, metabolism, pathology)
- Male
- Middle Aged
- Prognosis
- RNA, Messenger
(genetics, metabolism)
- Receptor, Interferon alpha-beta
(genetics, metabolism)
- Reverse Transcriptase Polymerase Chain Reaction
- Survival Rate
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