[Efficacy of absorbance ratio of ELISA antibodies [corrected] for hepatitis C virus of 3th generation in the prediction of viremia evaluated by PCR].

Abstract

BACKGROUND:

In order to decrease the burden of suffering and the costs derived from confirmatory molecular assays, a better strategy is badly needed to decrease the rate of false positive results of the enzyme-linked immunoassay (ELISA) for detection of hepatitis C virus (HCV) antibodies (Anti).

OBJECTIVE:

To establish the best cutoff of the S/CO rate in subjects with a positive result of a microparticule, third generation ELISA assay for Anti-HCV, for predicting viremia as detected by polymerase chain reaction (PCR) assay.

METHODS:

Using the result of the PCR assay as "gold standard", a ROC curve was build with the results of the S/CO rate values in subjects with a positive result for ELISA HCV assay.

RESULTS:

Fifty two subjects (30 male, 22 female, 40 +/- 12.5 years old) were included. Thirty four (65.3%) had a positive RNA HCV PCR assay. The area under the curve was 0.99 (95% CI: 0.98-1.0). The optimal cutoff for the S/CO rate was established in 29: sensitivity: 97%; specificity: 100%: PPV: 100%; NPV: 94%.

CONCLUSIONS:

Setting the cutoff of the S/CO in 29 results in a high predictive value for viremia as detected by PCR in subjects with a positive ELISA HVC assay. This knowledge may result in a better decision taking for the clinical follow up of those subjects with a positive result in the ELISA screening assay for HCV infection.

• Authors: Isidro Vázquez-Avila, Jorge Manuel Vera-Peralta, José Alvarez-Nemegyei, Otilia Rodríguez-Carvajal
• Journal: Revista de gastroenterologia de Mexico (Rev Gastroenterol Mex) 2007 Jan-Mar Vol. 72 Issue 1 Pg. 34-9 ISSN: 0375-0906 [Print] Mexico
• Vernacular Title: Eficacia del cociente de absorbancia de un análisis inmunoenzimático de tercera generación para anticuerpos al virus de hepatitis C en la predicción de viremia por reacción en cadena de polimerasa.
• PMID: 17685198 (Publication Type: English Abstract, Journal Article)

Chemical References

• Hepatitis C Antibodies

Topics

• Adult
• Enzyme-Linked Immunosorbent Assay
• Female
• Hepatitis C (blood, diagnosis)
• Hepatitis C Antibodies (blood)
• Humans
• Male
• Polymerase Chain Reaction
• Predictive Value of Tests
• Viremia (blood, diagnosis)