Abstract | BACKGROUND: PATIENTS AND METHODS: The objective of this study was to assess the role of eotaxin-1 in colorectal cancer (CRC). Levels of eotaxin-1 protein in CRC tissues (n = 86) and paired normal mucosa were compared after determination by ELISA. Plasma eotaxin-1 levels from CRC patients (n = 67) were also compared with controls (n = 103) using the same method. Moreover, a TaqMan system was used to evaluate the -384A>G eotaxin-1 gene variant in CRC patients (n = 241) and in a control group (n = 253). RESULTS:
Eotaxin-1 protein levels in colorectal tumours were significantly (P < 0.0001) higher than in normal tissue. Immunohistochemistry revealed eotaxin-1 expression in stromal cells such as fibroblasts and leukocytes of the CRC tissue. The plasma eotaxin-1 level in CRC patients was lower compared with controls (P < 0.0001). Patients with tumours classified as Dukes' stage B and C had lower levels than patients with tumours in Dukes' stage A. We found no difference in genotype distribution but noted a difference regarding allele distribution (P = 0.036) and a dominance of allele G in rectal cancer patients. CONCLUSION: The up-regulated eotaxin-1 protein expression in cancer tissue may reflect an eotaxin-1 mediated angiogenesis and/or a recruitment of leukocytes with potential antitumourigenic role. We noticed a dominance of the G allele in rectal cancer patients compared with colon cancer patients that was independent of eotaxin-1 expression.
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Authors | Dick Wågsäter, Sture Löfgren, Anders Hugander, Olaf Dienus, Jan Dimberg |
Journal | World journal of surgical oncology
(World J Surg Oncol)
Vol. 5
Pg. 84
(Jul 31 2007)
ISSN: 1477-7819 [Electronic] England |
PMID | 17672898
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
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Topics |
- Adenocarcinoma
(genetics, metabolism)
- Adult
- Aged
- Aged, 80 and over
- Chemokine CCL11
(genetics, metabolism)
- Colorectal Neoplasms
(genetics, metabolism)
- Enzyme-Linked Immunosorbent Assay
- Female
- Gene Frequency
- Genotype
- Humans
- Immunohistochemistry
- Male
- Middle Aged
- Polymorphism, Single Nucleotide
- Promoter Regions, Genetic
(drug effects, genetics)
- Up-Regulation
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