Abstract | OBJECTIVE: METHODS AND RESULTS: To determine the effect of cholesterol-enriched diet on cardiac performance and oxidative/nitrosative stress, wildtype and human apoB100 transgenic mice were fed a 2% cholesterol-enriched or a normal diet for 18 weeks. Serum cholesterol and LDL-cholesterol levels were significantly elevated only in the cholesterol-fed apoB100 transgenic mice, while serum triglycerides were increased in the transgenic mice fed a normal diet. Cholesterol-enriched diet significantly increased cardiac superoxide generation and NADPH oxidase expression and activity in apoB100 mice but not in wildtypes. Cardiac NO content and NO synthase activity did not change in either group. As assessed in isolated working hearts, aortic flow was significantly decreased only in apoB100 transgenic mice fed a cholesterol-enriched diet. The peroxynitrite decomposition catalyst FeTPPS attenuated the decrease in aortic flow in cholesterol-fed apoB100 mice. Immunohistochemistry showed elevated nitrotyrosine in the hearts of apoB100 mice fed the cholesterol-enriched diet. CONCLUSIONS:
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Authors | Tamás Csont, Erika Bereczki, Péter Bencsik, Gabriella Fodor, Anikó Görbe, Agnes Zvara, Csaba Csonka, László G Puskás, Miklós Sántha, Péter Ferdinandy |
Journal | Cardiovascular research
(Cardiovasc Res)
Vol. 76
Issue 1
Pg. 100-9
(Oct 01 2007)
ISSN: 0008-6363 [Print] England |
PMID | 17658498
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- 5,10,15,20-tetrakis(4-sulfonatophenyl)porphyrinato iron(III) chloride
- Apolipoprotein B-100
- Cholesterol, Dietary
- Metalloporphyrins
- Triglycerides
- Superoxides
- Peroxynitrous Acid
- 3-nitrotyrosine
- Tyrosine
- Cholesterol
- Nitric Oxide Synthase
- NADPH Oxidases
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Topics |
- Animals
- Apolipoprotein B-100
(genetics)
- Cholesterol
(blood)
- Cholesterol, Dietary
(administration & dosage)
- Female
- Heart Diseases
(metabolism)
- Humans
- Hypercholesterolemia
(metabolism)
- Immunohistochemistry
- Metalloporphyrins
(pharmacology)
- Mice
- Mice, Inbred C57BL
- Mice, Transgenic
- Models, Animal
- Myocardium
(chemistry, metabolism)
- NADPH Oxidases
(metabolism)
- Nitric Oxide Synthase
(metabolism)
- Oxidation-Reduction
- Oxidative Stress
- Peroxynitrous Acid
(analysis, metabolism)
- Regional Blood Flow
(drug effects)
- Superoxides
(metabolism)
- Triglycerides
(blood)
- Tyrosine
(analogs & derivatives, analysis, metabolism)
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