Cyclin B1 is commonly expressed in the cytoplasm of primary human acute myelogenous leukemia cells and serves as a leukemia-associated antigen associated with autoantibody response in a subset of patients.

Abstract

OBJECTIVES:

Aberrant expression of cyclin B1, a cell cycle regulator, is related to prognosis in various human malignancies. Additionally, cytoplasmic expression of cyclin B1 in epithelial malignancies is associated with a specific T-cell response and presumably also a humoral immune response. We therefore investigated (i) whether a similar expression pattern could be detected in native human acute myelogenous leukemia (AML) cells and (ii) whether cyclin B1 specific antibodies could be detected in AML.

METHODS:

AML cell expression of cyclin B1 was analyzed by flow cytometry and confocal microscopy. Humoral immune response in AML patient sera against cyclin B1 was analyzed by ELISA.

RESULTS:

AML cell expression of cyclin B1 was detected for all 42 patients; but the percentage of cyclin B1 positive cells showed a wide variation between patients. Confocal microscopy demonstrated that 32/42 (76%) patient samples showed abnormal cytoplasmic expression. Furthermore, the cytoplasmic expression was maintained after 14 d of in vitro culture and differentiation of the AML cells towards a dendritic cell phenotype. Cyclin B1 specific serum antibodies could be detected for seven of 65 patients with untreated AML.

CONCLUSIONS:

Our studies demonstrate that primary human AML cells show aberrant cytoplasmic expression of cyclin B1 for a majority of patients and a specific humoral immune response was also detected for a subset of patients with untreated leukemia.

• Authors: Elisabeth Ersvaer, Jian-Ying Zhang, Emmet McCormack, Astrid Olsnes, Nina Anensen, Eng M Tan, Bjørn Tore Gjertsen, Oystein Bruserud
• Journal: European journal of haematology (Eur J Haematol) Vol. 79 Issue 3 Pg. 210-25 (Sep 2007) ISSN: 0902-4441 [Print] England
• PMID: 17655707 (Publication Type: Journal Article, Research Support, N.I.H., Extramural)

Chemical References

• Antibodies, Neoplasm
• Antigens, Neoplasm
• CCNB1 protein, human
• Ccnb1 protein, mouse
• Cyclin B
• Cyclin B1

Topics

• Adult
• Aged
• Aged, 80 and over
• Animals
• Antibodies, Neoplasm (blood)
• Antibody Formation
• Antigens, Neoplasm
• Case-Control Studies
• Cell Differentiation
• Cell Nucleus (chemistry)
• Cyclin B (analysis, immunology)
• Cyclin B1
• Cytoplasm (chemistry)
• Dendritic Cells
• Enzyme-Linked Immunosorbent Assay
• Humans
• Leukemia, Myeloid, Acute (immunology, pathology)
• Mice
• Mice, SCID
• Middle Aged
• Neoplasm Transplantation
• Transplantation, Heterologous