[Effects of TCM treatment according to syndrome differentiation on expressions of nuclear factor-kappaB and gamma-glutamylcysteine synthetase in rats with chronic obstructive pulmonary disease of various syndrome types].

Abstract

OBJECTIVE:

To explore the mechanism of traditional Chinese medicine (TCM) treatment according to syndrome differentiation in treating chronic obstructive pulmonary disease (COPD) by observing the changes of nuclear factor-kappaB (NF-kappaB) and gamma-glutamylcysteine synthetase (gamma-GCS) expression levels in rats.

METHODS:

COPD model was established by modified method of combining fumigation and lipopolysaccharide (LPS) intra-tracheal dripping. Model rats were treated respectively for succesive 14 days according to their syndrome, that is, Xiaoqinglong Decoction to the rats of cold-phlegm accumulation in Fei, Maxing Shigan Decoction to those of heat-phlegm accumulation in Fei, Yupingfeng Decoction to those of Fei-qi deficiency, Liujunzi Decoction to those of Pi-qi deficiency, Renshen Gejie Decoction to those of Shen qi-deficiency. Besides, model rats in the model control group received 2mL normal saline daily, and no intervention was applied in the normal control group. The expression of gamma-GCS and NF-kappaB was detected by immunochemistry before and after treatment.

RESULTS:

Compared with that in the normal rats, the expressions of gamma-GCS and NF-kappaB in bronchial and alveolar epithelium of COPD rats before treatment were significantly higher, but the positive expression rates were lowered after treatment significantly (P<0.05).

CONCLUSION:

TCM treatment according to syndrome differentiation could rectify imbalance of oxidation/anti-oxidation and alleviate inflammatory reaction in COPD rats, thus to treat COPD effectively.

• Authors: Wei Zhang, Xin-Yue Zhang, Yu-Meng Shao
• Journal: Zhongguo Zhong xi yi jie he za zhi Zhongguo Zhongxiyi jiehe zazhi = Chinese journal of integrated traditional and Western medicine (Zhongguo Zhong Xi Yi Jie He Za Zhi) Vol. 27 Issue 5 Pg. 426-30 (May 2007) ISSN: 1003-5370 [Print] China
• PMID: 17650797 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Drugs, Chinese Herbal
• NF-kappa B
• sho-seiryu-to
• Glutamate-Cysteine Ligase

Topics

• Animals
• Diagnosis, Differential
• Drugs, Chinese Herbal (therapeutic use)
• Glutamate-Cysteine Ligase (biosynthesis)
• Immunohistochemistry
• Male
• Medicine, Chinese Traditional
• NF-kappa B (biosynthesis)
• Phytotherapy
• Pulmonary Disease, Chronic Obstructive (diagnosis, drug therapy, metabolism)
• Random Allocation
• Rats
• Rats, Wistar
• Syndrome