[Experimental study of 131I-labeled granulocyte macrophage colony-stimulating factor in SCID mouse-acute myeloid leukemia model].

Abstract

OBJECTIVE:

To observe the therapeutic efficacy of 131I-labeled granulocyte macrophage colony-stimulating factor (GM-SCF) in SCID mouse-acute myeloid leukemia model, and the relationship between dose and effect.

METHODS:

SCID-mouse acute myeloid leukemia model was established by injecting HL-60 cells through tail vein. GM-CSF was labeled with 131I by the chloramines-T method. SCID mice were randomly divided into 6 groups. Groups I, II and III treatment groups were given 9.25 x 10(5), 22.20 x 10(5) and 37.00 x 10(5) Bq of 131I-GM-CSF, respectively. Group IV was given 131I. Group V was given blending of 131I and GM-CSF. Group VI was control. Changes of HL-60 cells in blood and marrow, as well as white blood cells, red blood cells and platelets in blood were detected. Survival time of the SCID mice was calculated.

RESULT:

It was observed that WBC, HL-60 cells in blood and marrow were less in treatment groups than that in control groups, especially in groups II, III. After 2 weeks of treatment, BPC of II, III groups increased remarkably (P < 0.01). Survival time of the SCID mice was prolonged in treatment groups (P < 0.01), especially in group III, the longest survival time of 60 days.

CONCLUSION:

131I-GM-CSF could increase leukemic SCID mice survival rate. The therapeutic efficacy of low dose and mediate dose of 131I-GM-CSF is dose-dependent. 131I-GM-CSF is an effective radiation immunity therapy for leukemic mice.

• Authors: Yang-jia Deng, Shi-feng Lou, Yi-zhi Xu
• Journal: Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi (Zhonghua Xue Ye Xue Za Zhi) Vol. 28 Issue 1 Pg. 33-6 (Jan 2007) ISSN: 0253-2727 [Print] China
• PMID: 17649724 (Publication Type: English Abstract, Journal Article)

Chemical References

• Antineoplastic Agents
• Granulocyte-Macrophage Colony-Stimulating Factor

Topics

• Animals
• Antineoplastic Agents (therapeutic use)
• Dose-Response Relationship, Drug
• Female
• Granulocyte-Macrophage Colony-Stimulating Factor (therapeutic use)
• HL-60 Cells
• Humans
• Leukemia, Myeloid, Acute (drug therapy)
• Mice
• Mice, SCID
• Xenograft Model Antitumor Assays