Abstract | PURPOSE: We determined whether nephrocalcinosis is common and whether its detection is influenced by renal tissue processing. MATERIALS AND METHODS: Renal cortical and papillary tissue was obtained from the unaffected parts of 15 kidneys removed due to an oncological indication. The effect of tissue processing on the loss of crystals was studied in a kidney with nephrocalcinosis due to chronic pyelonephritis. Immediately frozen and formaldehyde fixed sections were analyzed by polarized light and Raman spectroscopy, and stained for calcium (Yasue) and hyaluronan. RESULTS: Although 13 of 15 snap-frozen sections from tumor kidneys contained birefringent particles (mean +/- SD 3.2 +/- 2.9 particles per cm(2)) in the renal tubules, this was not considered nephrocalcinosis because the crystals were not attached to the epithelial lining. Interstitial nephrocalcinosis was found on Yasue stain in 3 of 15 kidneys with tumor (20%). Calcium deposits were found in the papillary interstitium only, always together with hyaluronan. Formaldehyde fixed sections from the pyelonephritis kidney contained fewer renal tubular cell associated birefringent particles than immediately frozen sections (9.4 +/- 1.9 vs 41.6 +/- 1.2 per cm(2)). Particles were composed of calcium oxalate monohydrate (Yasue and Raman). CONCLUSIONS:
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Authors | Maxime T M Kummeling, Bas W D de Jong, Charlie Laffeber, Dirk-Jan Kok, Paul C M S Verhagen, Geert J L H van Leenders, Ron H N van Schaik, Christiaan S van Woerden, Anja Verhulst, Carl F Verkoelen |
Journal | The Journal of urology
(J Urol)
Vol. 178
Issue 3 Pt 1
Pg. 1097-103
(Sep 2007)
ISSN: 0022-5347 [Print] United States |
PMID | 17644134
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Topics |
- Female
- Frozen Sections
- Histological Techniques
- Humans
- Kidney Cortex
(pathology)
- Kidney Medulla
(pathology)
- Middle Aged
- Nephrocalcinosis
(pathology)
- Spectrum Analysis, Raman
- X-Ray Diffraction
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