Abstract |
The purpose of this study was to investigate the anticancer effect of aloe-emodin, an anthraquinone compound present in the leaves of Aloe vera, on two distinct human gastric carcinoma cell lines, AGS and NCI-N87. We demonstrate that aloe-emodin induced cell death in a dose- and time-dependent manner. Noteworthy is that the AGS cells were generally more sensitive than the NCI-N87 cells. Aloe-emodin caused the release of apoptosis-inducing factor and cytochrome c from mitochondria, followed by the activation of caspase-3, leading to nuclear shrinkage and apoptosis. In addition, exposure to aloe-emodin suppressed the casein kinase II activity in a time-dependent manner and was accompanied by a reduced phosphorylation of Bid, a downstream substrate of casein kinase II and a pro-apoptotic molecule. These preclinical studies suggest that aloe-emodin represents a suitable and novel chemotherapeutic drug candidate for the treatment of human gastric carcinoma.
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Authors | Sheng-Hsuan Chen, Kai-Yuan Lin, Chun-Chao Chang, Chia-Lang Fang, Chih-Ping Lin |
Journal | Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association
(Food Chem Toxicol)
Vol. 45
Issue 11
Pg. 2296-303
(Nov 2007)
ISSN: 0278-6915 [Print] England |
PMID | 17637488
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Anthraquinones
- Antineoplastic Agents, Phytogenic
- Apoptosis Inducing Factor
- BH3 Interacting Domain Death Agonist Protein
- BID protein, human
- Cytochromes c
- aloe emodin
- Casein Kinase II
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Topics |
- Anthraquinones
(pharmacology)
- Antineoplastic Agents, Phytogenic
(pharmacology)
- Apoptosis
(drug effects)
- Apoptosis Inducing Factor
- BH3 Interacting Domain Death Agonist Protein
(metabolism)
- Carcinoma
(drug therapy)
- Casein Kinase II
(metabolism)
- Cell Line, Tumor
- Cytochromes c
- Dose-Response Relationship, Drug
- Humans
- Phosphorylation
- Stomach Neoplasms
(drug therapy)
- Time Factors
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