Abstract | BACKGROUND:
Acrolein is a very reactive aldehyde present in cigarette smoke and endogenously generated by pathways such as lipid peroxidation and threonine metabolism by phagocytes. Acrolein has been shown to affect uptake of cholesterol by HDL. We hypothesized that acrolein could also have deleterious effects on paraoxonase 1 (PON-1) activity. We also determined whether free serum acrolein levels are higher in renal failure, and assessed whether they decrease after hemodialysis (HD) and whether this change correlates with increases in PON-1 activity. METHODS: RESULTS: We found that acrolein inhibits PON-1 activity in HDL in a time and concentration dependent fashion. Inhibition occurred at 40% at 0.5 mmol/l and was cancelled by cysteine but not by aminoguanidine or carnosine. We confirm that free serum acrolein levels are higher in chronic renal failure patients and demonstrate that they are partially removed by HD. Decrease in acrolein levels after dialysis correlate with increases in PON-1 activity (r=0.32, p 0.01). CONCLUSION:
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Authors | Alejandro Gugliucci, Nicole Lunceford, Eriko Kinugasa, Hiroaki Ogata, John Schulze, Satoshi Kimura |
Journal | Clinica chimica acta; international journal of clinical chemistry
(Clin Chim Acta)
Vol. 384
Issue 1-2
Pg. 105-12
(Sep 2007)
ISSN: 0009-8981 [Print] Netherlands |
PMID | 17632094
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Lipoproteins, HDL
- Acrolein
- Aryldialkylphosphatase
- Cysteine
- Acetylcysteine
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Topics |
- Acetylcysteine
(therapeutic use)
- Acrolein
(antagonists & inhibitors, blood, toxicity)
- Aged
- Aryldialkylphosphatase
(antagonists & inhibitors, blood)
- Cysteine
(chemistry, pharmacology)
- Female
- Humans
- Kidney Failure, Chronic
(diagnosis, drug therapy, therapy)
- Lipoproteins, HDL
(chemistry, metabolism)
- Male
- Middle Aged
- Renal Dialysis
- Treatment Outcome
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