[Inhibitive effect of troglitazone on TGF-beta(1) and fibronectin expression in human peritoneal mesothelial cells].

Abstract

OBJECTIVE:

To investigate the effect of the peroxisome proliferator activated receptor-gamma (PPAR-gamma) agonist troglitazone on TGF-beta(1) and fibronectin (Fn) expression in human peritoneal mesothelial cells (HPMCs).

METHODS:

HPMCs were cultured from human omentum by an enzyme digestion method, growing in medium containing 30 mmol/L D-glucose. TGF-beta(1) and Fn expression were measured in HPMCs in the presence and absence of 15 micromol/L troglitazone. The mRNA expressions of PPAR-gamma,TGF-beta(1) and Fn were determined by semi-quantification reverse transcriptive PCR (RT-PCR). The protein of TGF-beta(1) was determined by enzyme-linked immunosorbent assay (ELISA) and proteins of PPAR-gamma and Fn were determined by Western blot.

RESULTS:

The mRNA and protein expression of TGF-beta(1) and Fn were significantly increased in HPMCs stimulated with 30 mmol/L D-glucose compared with the control group with F12 media (P<0.01). Obvious decrease of TGF-beta(1) was found in troglitazone(15 micromol/L) treated group compared with group stimulated with 30 mmol/L D-glucose (P<0.05). Exposure of HPMCs to troglitazone reduced the Fn secretion (P<0.05).

CONCLUSION:

Troglitazone reduced the expression of TGF-beta(1) in HPMCs stimulated by 30mmol/L D-glucose, and reduced Fn production. PPAR-gamma agonists may have a specific role in ameliorating the course of progressive peritoneal fibrosis under long-term peritoneal dialysis states.

• Authors: Hong Liu, You-ming Peng, Fu-you Liu, Ying-hong Liu, Ling-yan Li, Jun Li, Xing Chen
• Journal: Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences (Zhong Nan Da Xue Xue Bao Yi Xue Ban) Vol. 32 Issue 3 Pg. 473-9 (Jun 2007) ISSN: 1672-7347 [Print] China
• PMID: 17611328 (Publication Type: Journal Article)

Chemical References

• Chromans
• Fibronectins
• PPAR gamma
• RNA, Messenger
• Thiazolidinediones
• Transforming Growth Factor beta1
• Troglitazone
• Glucose

Topics

• Blotting, Western
• Cells, Cultured
• Chromans (pharmacology)
• Dose-Response Relationship, Drug
• Enzyme-Linked Immunosorbent Assay
• Epithelial Cells (cytology, drug effects, metabolism)
• Fibronectins (biosynthesis, genetics)
• Glucose (pharmacology)
• Humans
• PPAR gamma (biosynthesis, genetics)
• Peritoneum (cytology)
• RNA, Messenger (biosynthesis, genetics)
• Reverse Transcriptase Polymerase Chain Reaction
• Thiazolidinediones (pharmacology)
• Transforming Growth Factor beta1 (biosynthesis, genetics)
• Troglitazone