Dopamine treatment in brain-dead rats mediates anti-inflammatory effects: the role of hemodynamic stabilization and D-receptor stimulation.

Abstract

Brain death (BD) is associated with profound inflammation in end-organs. Dopamine (DA) treatment reduces this inflammatory response, but the underlying mechanisms remain thus far largely unknown. In this study, we investigated if the anti-inflammatory effect of DA was related to hemodynamic stabilization and by which receptors it was mediated. BD was induced in F344 donor rats. DA was given either before BD for 24 h or after BD induction during a definite time. Adrenergic or D-receptor blockers were administered to inhibit the receptor stimulation mediated by DA. Hemodynamic changes were recorded and kidneys were harvested after 6 h of BD. Mean arterial pressure was completely normalized by DA treatment. DA pretreatment before BD induction and treatment during BD both significantly inhibited the monocyte infiltration. The anti-inflammatory as well as its blood pressure stabilizing effect was abrogated by concomitant application of adrenergic receptor blockers. In contrast, concomitant application of D-receptor blockers only abrogated the anti-inflammatory effect, but did not affect blood pressure stabilization. In contrast, pergolide and adrenergic receptor blockers completely normalized the blood pressure, but did not affect renal inflammation. Hence, DA might reduce BD-induced monocyte infiltration possibly by hemodynamic stabilization, D-receptor activation, or a combination of both.

• Authors: Simone Hoeger, Uwe Gottmann, Zhenzi Liu, Peter Schnuelle, Rainer Birck, Claude Braun, Fokko J van der Woude, Benito A Yard
• Journal: Transplant international : official journal of the European Society for Organ Transplantation (Transpl Int) Vol. 20 Issue 9 Pg. 790-9 (Sep 2007) ISSN: 0934-0874 [Print] Switzerland
• PMID: 17596177 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Adrenergic Antagonists
• Anti-Inflammatory Agents
• Cell Adhesion Molecules
• Dopamine Agonists
• Dopamine Antagonists
• Receptors, Dopamine
• Pergolide
• Heme Oxygenase-1
• Dopamine

Topics

• Adrenergic Antagonists (pharmacology)
• Animals
• Anti-Inflammatory Agents (pharmacology)
• Blood Pressure (drug effects)
• Brain Death (physiopathology)
• Cell Adhesion Molecules (blood)
• Cell Separation
• Dopamine (pharmacology)
• Dopamine Agonists (pharmacology)
• Dopamine Antagonists (pharmacology)
• Flow Cytometry
• Gene Expression (drug effects)
• Heme Oxygenase-1 (genetics)
• Leukocytes (metabolism)
• Male
• Nephritis (prevention & control)
• Pergolide (pharmacology)
• Rats
• Rats, Inbred F344
• Receptors, Dopamine (drug effects)