Abstract | PURPOSE: Suberonylanilide hydroxamic acid (SAHA) is an orally administered histone deacetylase inhibitor (HDACI) that has shown significant antitumor activity in a variety of tumor cells. To evaluate if SAHA has an activity against liver cancer, and with an aim to identify the altered cellular factors upon SAHA treatment, human HepG2 cancer cell line was used as a model, and proteomic approach was utilized to elucidate the molecular mechanisms underlying SAHA's antitumor activity. METHODS: Cell growth inhibition was measured by MTT method, and apoptosis was detected by means of flow cytometry analysis and TUNEL assay. Protein expression profiles were analyzed by 2-DE coupled with MALDI-Q-TOF MS/MS analysis. RESULTS: CONCLUSION: SAHA remarkably inhibited proliferation of HepG2 cancer cells, and induced apoptosis in vitro. Using proteomics approaches, a variety of differentially expressed proteins were identified in HepG2 cancer cells before and after treatment with SAHA. This study will enable a better understanding of the molecular mechanisms underlying SAHA-mediated antitumor effects at the protein level.
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Authors | Aiping Tong, Haiyuan Zhang, Zhengyu Li, Lantu Gou, Zhi Wang, Haiyan Wei, Minghai Tang, Shufang Liang, Lijuan Chen, Canhua Huang, Yuquan Wei |
Journal | Cancer chemotherapy and pharmacology
(Cancer Chemother Pharmacol)
Vol. 61
Issue 5
Pg. 791-802
(Apr 2008)
ISSN: 0344-5704 [Print] Germany |
PMID | 17593366
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antineoplastic Agents
- Histone Deacetylase Inhibitors
- Hydroxamic Acids
- RNA, Messenger
- Vorinostat
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Topics |
- Antineoplastic Agents
(pharmacology)
- Apoptosis
(drug effects)
- Cell Line, Tumor
- Cell Proliferation
(drug effects)
- Drug Screening Assays, Antitumor
- Electrophoresis, Gel, Two-Dimensional
- Flow Cytometry
- Histone Deacetylase Inhibitors
- Humans
- Hydroxamic Acids
(pharmacology)
- In Situ Nick-End Labeling
- Liver Neoplasms
(drug therapy)
- Proteomics
- RNA, Messenger
(metabolism)
- Reverse Transcriptase Polymerase Chain Reaction
- Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
- Tandem Mass Spectrometry
- Vorinostat
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