Abstract | OBJECTIVE: METHODS: Forty-five Sprague-Dawley rats were used in two experiments. In Experiment 1 (n = 20, including sham-operated controls), VEGF expression was analysed by Western blots in three different rat DAVF models: model I: common carotid artery-external jugular vein (CCA-EJV) anastomosis (n = 5); model II: sagittal sinus thrombosis and bipolar coagulation of the vein draining the transverse sinus (n = 5); model III: CCA-EJV anastomosis and bipolar coagulation of the vein draining the transverse sinus and sagittal sinus thrombosis to induce venous hypertension (n = 5). Based on the results of Experiment 1, Western blots were performed at weekly intervals 1, 2 and 3 weeks in Experiment 2 following induction of venous hypertension in model III (n = 5 at each time point and n = 5 sham controls); in addition, VEGF expression was immunohistochemically examined in the dura and the brain near the occluded sinus in five model III animals after 1 week. RESULTS: In Experiment 1, Western blot analysis showed barely detectable bands with molecular weights of 45 kD, corresponding to VEGF, in the sham group, but the highest level of VEGF was induced in model III, followed by models I and II (model III>model I>model II). In Experiment 2, the expression of VEGF peaked 1 week after induction of venous hypertension in model III, decreasing in a linear fashion over 2 and 3 weeks (week 1>weeks 2 and 3). The expression of immunoreactive VEGF was restricted in the connective tissue and the endothelial layer of the dura matter, cerebral cortical tissue and neurons of the basal ganglia. CONCLUSION:
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Authors | Yasushi Shin, Hiroyuki Nakase, Mitsutoshi Nakamura, Keiji Shimada, Noboru Konishi, Toshisuke Sakaki |
Journal | Neurological research
(Neurol Res)
Vol. 29
Issue 7
Pg. 727-33
(Oct 2007)
ISSN: 0161-6412 [Print] England |
PMID | 17588308
(Publication Type: Journal Article)
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Chemical References |
- Vascular Endothelial Growth Factor A
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Topics |
- Animals
- Brain
(metabolism, physiopathology)
- Brain Ischemia
(metabolism, physiopathology)
- Central Nervous System Vascular Malformations
(metabolism, physiopathology)
- Cerebrovascular Circulation
- Cranial Sinuses
(metabolism, pathology, physiopathology)
- Disease Models, Animal
- Endothelial Cells
(metabolism)
- Male
- Meningeal Arteries
(metabolism, pathology, physiopathology)
- Neovascularization, Pathologic
(metabolism, physiopathology)
- Rats
- Rats, Sprague-Dawley
- Up-Regulation
- Vascular Endothelial Growth Factor A
(metabolism)
- Venous Pressure
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