Innate immune responses in Swine resuscitated from severe traumatic hemorrhagic shock with hemoglobin-based oxygen carrier-201.

Abstract

Hemoglobin-based oxygen carrier-201 transports oxygen and improves survival in swine with hemorrhagic shock, but has potential to be immune activating. Herein, we evaluated HBOC-201's immune effects in swine with more severe hemorrhagic shock due to soft tissue injury and 55% blood volume catheter withdrawal over 15 minutes followed by fluid resuscitation at 20 minutes with HBOC-201, Hextend, or no treatment (NON) before hospital arrival. Survival rates were similar with HBOC-201 and Hextend (p > 0.05), but were higher than in (p = 0.007). There were no significant group differences in blood cell count, percentages of leukocyte sub-populations and immunophenotype (CD4:CD8 ratio), adhesion markers expression (neutrophil CD11b; monocyte or neutrophil CD49d) and apoptosis. There was a trend to higher plasma IL-10 in HBOC-201 and groups vs. Hextend. We conclude that in swine with severe controlled HS and soft tissue injury, immune responses are similar with resuscitation with HBOC-201 and Hextend.

• Authors: C Hall, N Malkevich, M Handrigan, C Vandermolen, F Aranaud, J Hong, F Dong, J Rice, N Philbin, S Ahlers, R McCarron, D Freilich, G McGwin, W S Flournoy, L Bruce Pearce
• Journal: Artificial cells, blood substitutes, and immobilization biotechnology (Artif Cells Blood Substit Immobil Biotechnol) Vol. 35 Issue 3 Pg. 259-74 ( 2007) ISSN: 1073-1199 [Print] England
• PMID: 17573626 (Publication Type: Journal Article, Research Support, U.S. Gov't, Non-P.H.S.)

Chemical References

• Blood Substitutes
• Cytokines
• Hemoglobins
• Hydroxyethyl Starch Derivatives
• HBOC 201

Topics

• Animals
• Apoptosis (immunology)
• Blood Pressure (physiology)
• Blood Substitutes (administration & dosage, pharmacokinetics)
• Cytokines (immunology)
• Disease Models, Animal
• Drug Evaluation, Preclinical
• Emergency Medical Services
• Fluid Therapy (methods)
• Hemoglobins (administration & dosage, pharmacokinetics)
• Hydroxyethyl Starch Derivatives (administration & dosage)
• Immunity, Innate (drug effects)
• Resuscitation (methods)
• Shock, Hemorrhagic (immunology, therapy)
• Swine
• Swine, Miniature
• T-Lymphocytes (immunology, pathology)