Abstract | BACKGROUND: This study examined the in vivo effects of ischemic preconditioning (IPC), ascorbic acid (AA), or a combination (IPC + AA) on the level of mitochondrial injury caused by hepatic ischemia/reperfusion (I/R). MATERIALS AND METHODS: A rat liver was preconditioned with 10 min of ischemia followed by 10 min of reperfusion, and then subjected to 90 min of ischemia followed by 5 h of reperfusion. The rats were pretreated with AA (100 mg/kg, i.v.) 5 min before the sustained ischemia. RESULTS: I/R increased the aminotransferase activity and level of mitochondrial lipid peroxidation, whereas it decreased the reduced glutathione: oxidized glutathione ratio. Either the IPC or AA pretreatment alone attenuated these changes, with the effect being enhanced by IPC + AA. The level of mitochondrial glutamate dehydrogenase, which is specifically located in the matrix, decreased after I/R but this was prevented by IPC + AA. Significant peroxide production was observed after 10 min of reperfusion after sustained ischemia. This change was attenuated by either IPC or AA alone and was further attenuated by IPC + AA. The mitochondria isolated after I/R were rapidly swollen, indicating an opening of the permeability transition pore. However, this was markedly reduced by IPC + AA. The hepatic ATP level was lower after I/R, which was restored by IPC alone and IPC + AA. CONCLUSIONS: Our findings suggest that IPC and AA synergistically reduce the level of mitochondrial damage during I/R as a result of decreased postischemic oxidant stress.
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Authors | Woo-Yong Lee, Jun-Seok Lee, Sun-Mee Lee |
Journal | The Journal of surgical research
(J Surg Res)
Vol. 142
Issue 1
Pg. 45-52
(Sep 2007)
ISSN: 0022-4804 [Print] United States |
PMID | 17559880
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antioxidants
- Peroxides
- Reactive Oxygen Species
- Malondialdehyde
- Glutamate Dehydrogenase
- Aspartate Aminotransferases
- Alanine Transaminase
- Glutathione
- Ascorbic Acid
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Topics |
- Alanine Transaminase
(blood)
- Animals
- Antioxidants
(therapeutic use)
- Ascorbic Acid
(therapeutic use)
- Aspartate Aminotransferases
(blood)
- Energy Metabolism
(drug effects, physiology)
- Glutamate Dehydrogenase
(blood)
- Glutathione
(metabolism)
- Ischemic Preconditioning
(methods)
- Liver
(injuries, metabolism, pathology)
- Male
- Malondialdehyde
(metabolism)
- Mitochondria, Liver
(drug effects, metabolism, physiology)
- Oxidative Stress
(drug effects)
- Peroxides
(metabolism)
- Rats
- Rats, Sprague-Dawley
- Reactive Oxygen Species
(metabolism)
- Reperfusion Injury
(metabolism, pathology, therapy)
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