Abstract | PURPOSE: EXPERIMENTAL DESIGN: RESULTS: All flash-frozen carcinosarcoma biopsies (12 of 12) and short-term carcinosarcoma cell lines evaluated overexpressed claudin-3 and claudin-4 by quantitative reverse transcription-PCR. Membranous immunoreactivity for claudin-4 protein expression was documented in 80% (20 of 25) of primary tumors and 100% (6 of 6) of the metastatic carcinosarcomas, whereas negligible staining was found in normal endometrial cells. Regardless of their resistance to chemotherapeutic agents, all short-term carcinosarcoma cell lines tested died within 1 h of exposure to 3.3 microg/mL of CPE in vitro. Intratumoral injections of well-tolerated doses of CPE in large s.c. carcinosarcoma xenografts led to large areas of tumor cell necrosis and tumor disappearance in all treated animals. CONCLUSIONS:
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Authors | Alessandro D Santin, Stefania Bellone, Eric R Siegel, Jesse K McKenney, Maria Thomas, Juan J Roman, Alexander Burnett, Germana Tognon, Elisabetta Bandiera, Sergio Pecorelli |
Journal | Clinical cancer research : an official journal of the American Association for Cancer Research
(Clin Cancer Res)
Vol. 13
Issue 11
Pg. 3339-46
(Jun 01 2007)
ISSN: 1078-0432 [Print] United States |
PMID | 17545541
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- CLDN3 protein, human
- CLDN4 protein, human
- Claudin-3
- Claudin-4
- Cldn3 protein, mouse
- Cldn4 protein, mouse
- Enterotoxins
- Membrane Proteins
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Topics |
- Aged
- Aged, 80 and over
- Animals
- Carcinosarcoma
(metabolism, microbiology)
- Claudin-3
- Claudin-4
- Clostridium perfringens
(metabolism)
- Enterotoxins
(metabolism)
- Female
- Gene Expression Regulation, Neoplastic
- Humans
- Membrane Proteins
(biosynthesis)
- Mice
- Mice, SCID
- Middle Aged
- Neoplasm Transplantation
- Uterine Neoplasms
(metabolism, microbiology)
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