Abstract | PURPOSE: METHODS: A total of 30 NZW rabbits each were intrastromally inoculated in both eyes with approximately 1000 CFU of SM and PA. By E-test, the minimum inhibitory concentrations (MICs; microg/mL) for SM were gatifloxacin (0.125), ciprofloxacin (0.047), and tobramycin (1.5), and for PA were gatifloxacin (0.125), ciprofloxacin (0.19), and tobramycin (0.5). After 16 hours, the rabbits were divided into 4 treatment groups: (1) Zymar, (2) Ciloxan, (3) fortified tobramycin, and (4) saline control. One drop was instilled in both eyes every 15 minutes for 5 doses and then every 30 minutes for 14 doses. One hour after the final treatment, the animals were euthanized, and bacterial colony counts from the corneas were determined. RESULTS: For SM, Zymar and Ciloxan significantly reduced (P < 0.001, ANOVA) the colony counts compared with tobramycin and saline control. Zymar was more effective than Ciloxan (P < 0.001, ANOVA). For PA, all antibiotics reduced equivalently the colony counts compared with the saline control (P = 0.005, ANOVA). CONCLUSIONS: The enhanced Gram-positive activity of gatifloxacin is not associated with any decreased Gram-negative activity in vivo. Zymar may prove useful for SM and PA keratitis.
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Authors | Francis S Mah, Eric G Romanowski, Regis P Kowalski, Kathleen A Yates, Y Jerold Gordon |
Journal | Cornea
(Cornea)
Vol. 26
Issue 5
Pg. 585-8
(Jun 2007)
ISSN: 0277-3740 [Print] United States |
PMID | 17525656
(Publication Type: Comparative Study, Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
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Chemical References |
- Anti-Infective Agents
- Fluoroquinolones
- Ciprofloxacin
- Gatifloxacin
- Tobramycin
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Topics |
- Animals
- Anti-Infective Agents
(therapeutic use)
- Ciprofloxacin
(therapeutic use)
- Colony Count, Microbial
- Corneal Ulcer
(drug therapy, microbiology)
- Disease Models, Animal
- Eye Infections, Bacterial
(drug therapy, microbiology)
- Female
- Fluoroquinolones
(therapeutic use)
- Gatifloxacin
- Pseudomonas Infections
(drug therapy, microbiology)
- Pseudomonas aeruginosa
(drug effects, isolation & purification)
- Rabbits
- Serratia Infections
(drug therapy, microbiology)
- Serratia marcescens
(drug effects, isolation & purification)
- Tobramycin
(therapeutic use)
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