Abstract | PURPOSE: METHODS: Mouse corneal epithelial cells and keratocytes were immortalized using SV40T antigen viral infection of primary cultures. The immortalized epithelial cells and keratocytes were cloned and characterized using antibodies to keratin, vimentin, integrin alpha5beta1, and alpha-smooth muscle actin. Basic fibroblast growth factor pellets were implanted to induce corneal neovascularization. The eyes of wild-type, ephrinB2(tlacZ/+), and EphB4(tlacZ/+) heterozygous mice were harvested and sectioned 7 days after pellet implantation. Confocal immunohistochemistry was performed to compare the expression of the Eph/ephrinA family (EphA1-8, ephrinA1-5) and Eph/ephrinB family (EphB1-4, EphB6 ephrinB1-3). RESULTS: EphA1, EphA3, ephrinA1, ephrinA2, EphB1, EphB4, ephrinB1, and ephrinB2 were detected in wild-type mouse corneal epithelial cells and keratocytes. EphA2 was immunolocalized only in epithelial cells. Also, EphA3, ephrinA1, EphB1, EphB4, and ephrinB1 were immunolocalized to the corneal epithelium and stroma. In the vascularized corneas, ephrinB1 was immunolocalized mainly to the keratocytes around the vessels, and ephrinB2, EphB1, and EphB4 were colocalized mainly with CD31 to the vascular endothelial cells. CONCLUSIONS:
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Authors | Takashi Kojima, Tae-Young Chung, Jin-Hong Chang, Rony Sayegh, Fabio H Casanova, Dimitri T Azar |
Journal | Cornea
(Cornea)
Vol. 26
Issue 5
Pg. 569-78
(Jun 2007)
ISSN: 0277-3740 [Print] United States |
PMID | 17525654
(Publication Type: Comparative Study, Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
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Chemical References |
- Actins
- Ephrins
- Integrin alpha5beta1
- Ligands
- Vimentin
- Keratins
- Receptors, Eph Family
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Topics |
- Actins
(metabolism)
- Animals
- Cell Line
- Corneal Neovascularization
(metabolism)
- Corneal Stroma
(cytology)
- Ephrins
(metabolism)
- Epithelium, Corneal
(metabolism)
- Fibroblasts
(metabolism)
- Fluorescent Antibody Technique, Indirect
- Integrin alpha5beta1
(metabolism)
- Keratins
(metabolism)
- Ligands
- Mice
- Mice, Inbred C57BL
- Mice, Transgenic
- Microscopy, Confocal
- Receptors, Eph Family
(metabolism)
- Vimentin
(metabolism)
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