Abstract |
Many patients surviving vasculitis are prone to accelerated atherosclerosis and often have enhanced levels of antibodies to oxidized low-density lipoprotein ( oxLDL). To measure anti- oxLDL antibodies, oxidation of LDL is achieved with copper (Cu) or malondialdehyde (MDA). Because, in vivo, LDL may be oxidized with myeloperoxidase (MPO) or its product hypochlorite, we measured anti- hypochlorite LDL antibodies in patients with vasculitis, haemodialysis patients and healthy controls. A newly developed enzyme-linked immunosorbent assay (ELISA) was used to detect antibodies to oxLDL as modified by hypochlorite. Results are compared with data obtained by standard LDL oxidation using MDA- LDL or Cu- LDL as substrate. Results were compared between anti-neutrophil cytoplasmic antibodies ( ANCA)-associated vasculitis (AAV) patients (n = 93), haemodialysis (HD) patients (n = 59) and healthy controls (HC; n = 43). Furthermore, patients with MPO- ANCA-associated vasculitis (n = 47) were compared with patients with proteinase 3 (PR3)-ANCA associated vasculitis (n = 46). Optimal cut-off points were determined by receiver operator characteristic (ROC) curve analysis. Anti- oxLDL antibodies are enhanced in AAV patients (MDA- LDL and hypochlorite- LDL) and in HD patients ( hypochlorite- LDL), when compared to HC. Furthermore, patients with MPO- ANCA-associated vasculitis had higher levels of antibodies to hypochlorite- LDL than patients with PR3-ANCA-associated vasculitis. Our newly developed assay, in which hypochlorite- LDL is used as substrate, seems a more sensitive assay than traditional assays to measure oxLDL antibodies. Furthermore, our results suggest that enhanced MPO-mediated LDL oxidation occurs in patients with MPO- ANCA.
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Authors | M C Slot, R Theunissen, P van Paassen, J G M C Damoiseaux, J W Cohen Tervaert, Limburg Nephrology Working Group |
Journal | Clinical and experimental immunology
(Clin Exp Immunol)
Vol. 149
Issue 2
Pg. 257-64
(Aug 2007)
ISSN: 0009-9104 [Print] England |
PMID | 17521320
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antibodies, Antineutrophil Cytoplasmic
- Autoantibodies
- Lipoproteins, LDL
- oxidized low density lipoprotein
- Malondialdehyde
- Hypochlorous Acid
- Copper
- Peroxidase
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Topics |
- Adult
- Aged
- Antibodies, Antineutrophil Cytoplasmic
(analysis)
- Autoantibodies
(blood)
- Autoimmune Diseases
(enzymology, immunology)
- Copper
- Enzyme-Linked Immunosorbent Assay
(methods)
- Female
- Glomerulonephritis
(enzymology, immunology)
- Humans
- Hypochlorous Acid
- Lipoproteins, LDL
(immunology)
- Male
- Malondialdehyde
- Middle Aged
- Oxidation-Reduction
- Peroxidase
(metabolism)
- Renal Dialysis
- Vasculitis
(enzymology, immunology)
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