Complementation of the oxidatively damaged DNA repair defect in Cockayne syndrome A and B cells by Escherichia coli formamidopyrimidine DNA glycosylase.

Abstract	Repair of the oxidized purine 8-oxo-7,8-dihydroguanine (8-oxoGua) is inefficient in cells belonging to the B complementation group of Cockayne syndrome (CS-B), a developmental and neurological disorder characterized by defective transcription-coupled repair. We show here that cells belonging to the A complementation group (CS-A) are also defective in repair of 8-oxoGua and we demonstrate that expression of the Escherichia coli formamidopyrimidine DNA glycosylase (FPG) completely corrects the repair deficiency in both CS-A and CS-B cells. Phenotypically, CS-A cells are normally sensitive to toxicity and micronuclei induced by the oxidizing agent potassium bromate. CS-B cells display sensitivity to elevated concentrations of potassium bromate but this is not compensated by FPG expression, suggesting toxicity of lesions that are not FPG substrates. The data indicate that 8-oxoGua is not a major toxic and clastogenic lesion in CS cells.
Authors	Monica Ropolo, Paolo Degan, Mara Foresta, Mariarosaria D'Errico, Denise Lasigliè, Eugenia Dogliotti, Gianluigi Casartelli, Simonetta Zupo, Alessandro Poggi, Guido Frosina
Journal	Free radical biology & medicine (Free Radic Biol Med) Vol. 42 Issue 12 Pg. 1807-17 (Jun 15 2007) ISSN: 0891-5849 [Print] United States
PMID	17512460 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References	Bromates Carcinogens potassium bromate DNA-Formamidopyrimidine Glycosylase
Topics	Adolescent Adult Aged Aged, 80 and over Bromates (pharmacology) Carcinogens (pharmacology) Cell Survival (drug effects) Cells, Cultured (drug effects) Cockayne Syndrome (genetics) Colony-Forming Units Assay DNA Damage DNA Repair DNA-Formamidopyrimidine Glycosylase (genetics, metabolism) Escherichia coli (enzymology) Female Fibroblasts (drug effects) Genetic Complementation Test Genetic Vectors Humans Kidney (metabolism, pathology) Male Micronucleus Tests Transcription, Genetic Urinary Bladder Neoplasms (metabolism, pathology)

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