Abstract |
Interphase fluorescence in situ hybridisation (FISH) is a sensitive diagnostic tool used for the detection of alterations in the genome on cell-by-cell basis. However, the cost-per-test and the technical complexity of current FISH protocols have slowed its widespread utilisation in clinical settings. For many cancers, the lack of a cost-effective and informative diagnostic method has compromised the quality of life for patients. We present the first demonstration of a microchip-based FISH protocol, coupled with a novel method to immobilise peripheral blood mononuclear cells inside microfluidic channels. These first on-chip implementations of FISH allow several chromosomal abnormalities associated with multiple myeloma to be detected with a ten-fold higher throughput and 1/10-th the reagent consumption of the traditional slide-based method. Moreover, the chip test is performed within hours whereas the conventional protocol required days. In addition, two on-chip methods to enhance the hybridisation aspects of FISH have been examined: mechanical and electrokinetic pumping. Similar agitation methods have led to significant improvements in hybridisation efficiency with DNA microarray work, but with this cell-based method the benefits were moderate. On-chip FISH technology holds promise for sophisticated and cost-effective screening of cancer patients at every clinic visit.
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Authors | V J Sieben, C S Debes Marun, P M Pilarski, G V Kaigala, L M Pilarski, C J Backhouse |
Journal | IET nanobiotechnology
(IET Nanobiotechnol)
Vol. 1
Issue 3
Pg. 27-35
(Jun 2007)
ISSN: 1751-8741 [Print] United States |
PMID | 17506594
(Publication Type: Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
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Topics |
- Cytogenetic Analysis
(instrumentation, methods)
- DNA, Neoplasm
(genetics)
- Equipment Design
- Equipment Failure Analysis
- Humans
- In Situ Hybridization, Fluorescence
(instrumentation, methods)
- Microfluidic Analytical Techniques
(instrumentation, methods)
- Multiple Myeloma
(diagnosis, genetics)
- Reproducibility of Results
- Sensitivity and Specificity
- Tumor Cells, Cultured
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