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Evaluation of T cell immune responses in multi-drug-resistant tuberculosis (MDR-TB) patients to Mycobacterium tuberculosis total lipid antigens.

Abstract
Mycobacterium tuberculosis lipid antigens produce significant T cell responses in healthy tuberculin reactor [purified protein derivative (PPD-positive] individuals. In the present study, proliferation and interferon (IFN)-gamma/interleukin (IL)-4 responses were analysed to M. tuberculosis total lipid antigens in T lymphocytes from 25 patients with multi-drug-resistant tuberculosis (MDR-TB). The obtained results were compared with those of 30 asymptomatic healthy PPD-positive and 30 healthy tuberculin skin test negative (PPD-negative) subjects. Peripheral blood mononuclear cells (PBMCs) and T cells (CD4(+) and CD8(+)) were stimulated using autologous immature dendritic cells. Proliferation responses were assessed using 3-{4,5-dimethylthiazol-2-yl}-2,5 diphenyl tetrazolium bromide (MTT). IFN-gamma/IL-4 concentrations in the supernatant of the CD4(+) and CD8(+)T cells were measured by enzyme-linked immunosorbent assay. Proliferation assay showed that the peripheral blood mononuclear cells and CD4(+) T cells from the MDR-TB patients responded significantly less to the M. tuberculosis total lipid antigens than to the CD4(+) T cells in the PPD-positive subjects. Total lipid antigen-specific proliferative responses in the CD8(+) T cells from the MDR-TB patients were minimally detected and the responses were similar to those of the PPD-positive subjects. IFN-gamma production by the CD4(+) T cells stimulated by total lipid antigens from the MDR-TB patients was decreased significantly compared with the PPD-positive individuals, whereas IL-4 production in the patients was elevated. IFN-gamma and IL-4 production in the CD8(+) T cells of the MDR-TB patients was similar to those of the PPD-positive subjects. In conclusion, it is suggested that stimulated CD4(+) T cells by M. tuberculosis total lipid antigens may be shifted to T helper 2 responses in MDR-TB patients.
AuthorsA S Shahemabadi, A Zavaran Hosseini, S Shaghsempour, M R Masjedi, M Rayani, M Pouramiri
JournalClinical and experimental immunology (Clin Exp Immunol) Vol. 149 Issue 2 Pg. 285-94 (Aug 2007) ISSN: 0009-9104 [Print] England
PMID17490401 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Antigens, Bacterial
  • Lipids
  • Tuberculin
  • Mycobacterium tuberculosis antigens
  • Interleukin-4
  • Interferon-gamma
Topics
  • Adult
  • Antigens, Bacterial (immunology)
  • CD4-Positive T-Lymphocytes (immunology)
  • CD8-Positive T-Lymphocytes (immunology)
  • Cell Proliferation
  • Electrophoresis, Polyacrylamide Gel (methods)
  • Female
  • Humans
  • Immunity, Cellular
  • Interferon-gamma (biosynthesis)
  • Interleukin-4 (biosynthesis)
  • Lipids (immunology)
  • Male
  • Middle Aged
  • T-Lymphocyte Subsets (immunology)
  • Tuberculin (immunology)
  • Tuberculin Test
  • Tuberculosis, Multidrug-Resistant (immunology)

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