Mycobacterium tuberculosis
lipid antigens produce significant T cell responses in healthy
tuberculin reactor [purified
protein derivative (
PPD-positive] individuals. In the present study, proliferation and
interferon (IFN)-gamma/
interleukin (IL)-4 responses were analysed to M.
tuberculosis total
lipid antigens in T lymphocytes from 25 patients with
multi-drug-resistant tuberculosis (MDR-TB). The obtained results were compared with those of 30 asymptomatic healthy
PPD-positive and 30 healthy
tuberculin skin test negative (
PPD-negative) subjects. Peripheral blood mononuclear cells (PBMCs) and T cells (CD4(+) and CD8(+)) were stimulated using autologous immature dendritic cells. Proliferation responses were assessed using 3-{4,5-dimethylthiazol-2-yl}-2,5
diphenyl tetrazolium
bromide (MTT). IFN-gamma/IL-4 concentrations in the supernatant of the CD4(+) and CD8(+)T cells were measured by
enzyme-linked
immunosorbent assay. Proliferation assay showed that the peripheral blood mononuclear cells and CD4(+) T cells from the MDR-TB patients responded significantly less to the M.
tuberculosis total
lipid antigens than to the CD4(+) T cells in the
PPD-positive subjects. Total
lipid antigen-specific proliferative responses in the CD8(+) T cells from the MDR-TB patients were minimally detected and the responses were similar to those of the
PPD-positive subjects. IFN-gamma production by the CD4(+) T cells stimulated by total
lipid antigens from the MDR-TB patients was decreased significantly compared with the
PPD-positive individuals, whereas IL-4 production in the patients was elevated. IFN-gamma and IL-4 production in the CD8(+) T cells of the MDR-TB patients was similar to those of the
PPD-positive subjects. In conclusion, it is suggested that stimulated CD4(+) T cells by M.
tuberculosis total
lipid antigens may be shifted to T helper 2 responses in MDR-TB patients.