Condensin mutations and abnormal chromosomal structures in pyothorax-associated lymphoma.
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| Abstract |
Transfer of genetic information during mitosis is accurately conducted by proper condensation and segregation of chromosomes, for which condensins play a central role. Both condensin I and II have common structural maintenance of chromosomes subunits, named hCAP-C and hCAP-E. Pyothorax-associated lymphoma (PAL) is a non-Hodgkin's lymphoma developing in the pleural cavity of patients with long-standing pyothorax. Mutations of hCAP-C and hCAP-E were investigated in 24 leukemia-lymphoma cell lines including eight PAL cell lines, and their influences in chromosome morphology were evaluated. Heterozygous point mutations within hCAP-C were found in two PAL cell lines and corresponding tumor samples (OPL-3 and OPL-7). Deletion of exon 24 within hCAP-E and a point mutation at the donor splice site of intron 24 were detected in OPL-5 and original tumor samples. OPL-5 showed an extensive reduction in expression of not only hCAP-E but also hCAP-C proteins. OPL-5 occasionally showed the chromosome bridge in anaphase and telophase, indicating that segregation is not accurate. OPL-7 showed reduced hCAP-C protein expression, abnormality in chromosome length and width, and abnormal aggregates of hCAP-C protein. These findings indicated that condensin gene alteration might play a role in genome instability, which accelerates the accumulation of other gene alterations in PAL.
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| Authors | Maria Francisca Ham, Tetsuya Takakuwa, Nur Rahadiani, Kristianti Tresnasari, Hiroo Nakajima, Katsuyuki Aozasa |
| Journal | Cancer science (Cancer Sci) Vol. 98 Issue 7 Pg. 1041-7 (Jul 2007) ISSN: 1347-9032 [Print] England |
| PMID | 17488335 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't) |
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