Abstract | OBJECTIVE: METHODS: The hyperplastic scar from one female burn patient with 1% TBSA deep-partial thickness burns were implanted into subcutaneous skin of scapular region of 48 nude mice. Three weeks later, the nude mice were divide into large dose (LA) , medium dose (MD) , small dose (SD) and control groups, with 12 mice in each group. The mice in LA,MD and SD groups were injected with 200 microl of 15,10, 5 microg/ml VEGF monoclonal antibody diluted in 0.01 mol/L PBS, respectively in the scar twice a week for 3 weeks, while those in C group were injected with equal amount of 0. 01 mol/L PBS. The area and volume of the scar in each group were calculated and histological changes were observed, and the expression of collagen type I mRNA and its protein in each group were determined 3 days after treatment. RESULTS: The volume of scar in LA, MD, SD and C groups were (55.3 +/-4.1, 67.9 +/-5.7, 78.9 +/-5.5, 85.0 +7.3) mm(3), respectively. Compared with that in C group, the volume of the scar were significantly decreased in AD and MD groups ( P <0.05). A few number of vessels and fibroblasts were observed in LD, MD groups, with decreased number of collagen fibers arranged in order. Compared with that in C group ,The expression of procollagen type I mRNA and its protein in C group was obviously higher than those in LD and MD groups ( P < 0. 05) , but it was similar to those in SD group. CONCLUSION:
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Authors | Yi-gang Yue, Chang-wen Jiang, Pei-ying Li, Si Zhou |
Journal | Zhonghua shao shang za zhi = Zhonghua shaoshang zazhi = Chinese journal of burns
(Zhonghua Shao Shang Za Zhi)
Vol. 22
Issue 6
Pg. 427-30
(Dec 2006)
ISSN: 1009-2587 [Print] China |
PMID | 17438687
(Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antibodies, Monoclonal
- Collagen Type I
- RNA, Messenger
- VEGFA protein, human
- Vascular Endothelial Growth Factor A
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Topics |
- Adult
- Animals
- Antibodies, Monoclonal
(therapeutic use)
- Cicatrix, Hypertrophic
(metabolism, therapy)
- Collagen Type I
(metabolism)
- Disease Models, Animal
- Female
- Gene Expression
- Humans
- Hyperplasia
(metabolism, therapy)
- Mice
- Mice, Inbred BALB C
- Mice, Nude
- Neovascularization, Pathologic
(metabolism)
- RNA, Messenger
(metabolism)
- Vascular Endothelial Growth Factor A
(immunology)
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