The protective role of heme oxygenase-1 induction on testicular tissues after testicular torsion and detorsion.

Abstract

PURPOSE:

Testicular torsion-detorsion has been identified as an ischemia-reperfusion type of injury. We elucidated the protective role of heme oxygenase-1 super induction on testicular torsion-detorsion injury.

MATERIALS AND METHODS:

Adult male Sprague-Dawley rats were randomly allocated to undergo testicular torsion-detorsion, immediately followed by injection of normal saline, the heme oxygenase-1 inducer hemin or hemin plus the heme oxygenase-1 inhibitor tin protoporphyrin. Another set of rats that underwent sham operation, immediately followed by injection of normal saline, hemin or hemin plus tin protoporphyrin, served as controls. Testes were harvested 4 and 24 hours after detorsion, respectively, in the experimental groups or at comparable time points in the control groups.

RESULTS:

Histological evaluation confirmed that torsion-detorsion caused significant testicular tissue injury. Torsion-detorsion also caused significant increases in the testicular levels of nitric oxide, malondialdehyde, myeloperoxidase activity and heme oxygenase-1. The heme oxygenase-1 inducer hemin significantly enhanced the heme oxygenase-1 expression induced by torsion-detorsion and in turn attenuated testicular injury, and increases in nitric oxide, malondialdehyde and myeloperoxidase activity. In addition, the protective effects of hemin were significantly offset by the heme oxygenase-1 inhibitor tin protoporphyrin.

CONCLUSIONS:

Super induction of heme oxygenase-1 protects testes from torsion-detorsion injury.

• Authors: Stone Yang, Hung-Jen Shih, Yung-Chiong Chow, Pei-Shan Tsai, Tao-Yeuan Wang, Paulus S Wang, Chun-Jen Huang
• Journal: The Journal of urology (J Urol) Vol. 177 Issue 5 Pg. 1928-33 (May 2007) ISSN: 0022-5347 [Print] United States
• PMID: 17437850 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Biomarkers
• Enzyme Inhibitors
• Metalloporphyrins
• Protoporphyrins
• Nitric Oxide
• Malondialdehyde
• Hemin
• tin protoporphyrin IX
• Peroxidase
• Heme Oxygenase-1

Topics

• Animals
• Biomarkers (metabolism)
• Disease Models, Animal
• Enzyme Inhibitors (administration & dosage)
• Heme Oxygenase-1 (drug effects, metabolism)
• Hemin (administration & dosage)
• Immunoblotting
• Injections, Intraperitoneal
• Male
• Malondialdehyde (metabolism)
• Metalloporphyrins (administration & dosage)
• Nitric Oxide (metabolism)
• Oxidative Stress (drug effects)
• Peroxidase (metabolism)
• Protoporphyrins (administration & dosage)
• Rats
• Rats, Sprague-Dawley
• Reperfusion Injury (metabolism, pathology, prevention & control)
• Spectrophotometry
• Spermatic Cord (metabolism, pathology)
• Spermatic Cord Torsion (metabolism, pathology, prevention & control)
• Treatment Outcome