Construction of a high-density and high-resolution human chromosome X array for comparative genomic hybridization analysis.

Abstract

The human chromosome X is closely associated with congenital disorders and mental retardation (MR), because it contains a significantly higher number of genes than estimated from the proportion in the human genome. We constructed a high-density and high-resolution human chromosome X array (X-tiling array) for comparative genomic hybridization (CGH). The array contains a total of 1,001 bacterial artificial chromosome (BACs) throughout chromosome X except pseudoautosomal regions and two BACs specific for Y. In four hybridizations using DNA samples from healthy males, the ratio of each spotted DNA was scattered between -3SD and 3SD, corresponding to a log(2) ratio of -0.35 and 0.35, respectively. Using DNA samples from patients with known congenital disorders, our X-tiling array was proven to discriminate one-copy losses and gains together with their physical sizes, and also to estimate the percentage of a mosaicism in a patient with mos 45,X[13]/46,X,r(X)[7]. Furthermore, array-CGH in a patient with atypical Schinzel-Giedion syndrome disclosed a 1.1-Mb duplication at Xq22.3 including a part of the IL1RAPL2 gene as a likely causative aberration. The results indicate our in-house X-tiling array to be useful for the identification of cryptic copy-number aberrations containing novel genes responsible for diseases such as congenital disorders and X-linked MR.

• Authors: Shin Hayashi, Shozo Honda, Maki Minaguchi, Yoshio Makita, Nobuhiko Okamoto, Rika Kosaki, Torayuki Okuyama, Issei Imoto, Shuki Mizutani, Johji Inazawa
• Journal: Journal of human genetics (J Hum Genet) Vol. 52 Issue 5 Pg. 397-405 ( 2007) ISSN: 1434-5161 [Print] England
• PMID: 17406783 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• IL1RAPL2 protein, human
• Interleukin-1 Receptor Accessory Protein

Topics

• Chromosomes, Artificial, Bacterial
• Chromosomes, Human, X
• Gene Dosage
• Genetic Diseases, X-Linked (genetics)
• Humans
• Interleukin-1 Receptor Accessory Protein (genetics)
• Male
• Mental Retardation, X-Linked (genetics)
• Microarray Analysis (methods)
• Nucleic Acid Hybridization