Abstract | PURPOSE: EXPERIMENTAL DESIGN: RESULTS: We found that VES induced IGFBP-3 mRNA and protein levels in human prostate cancer cell lines. Knockdown of IGFBP-3 by small interfering RNA attenuated VES-induced IGFBP-3 expression and VES-mediated antiproliferative and proapoptotic functions. Furthermore, administration of VES resulted in a significant therapeutic effect on LNCaP and PC3 xenografts and a preventive effect on tumorigenic progression in the TRAMP model without overt toxicity. Notably, the therapeutic and preventive efficacy of VES correlated with increased accumulation of IGFBP-3 in mouse serum as well as in the xenograft tumors and TRAMP prostate samples. Consequently, reduced proliferation and induced apoptosis were witnessed. CONCLUSIONS: VES mediates its therapeutic and preventive effects against prostate cancer at least partially through up-regulating IGFBP-3, which inhibits cell proliferation and promotes cell apoptosis.
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Authors | Yi Yin, Jing Ni, Ming Chen, Matthew A DiMaggio, Yinglu Guo, Shuyuan Yeh |
Journal | Clinical cancer research : an official journal of the American Association for Cancer Research
(Clin Cancer Res)
Vol. 13
Issue 7
Pg. 2271-80
(Apr 01 2007)
ISSN: 1078-0432 [Print] United States |
PMID | 17404112
(Publication Type: Journal Article, Research Support, N.I.H., Extramural)
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Chemical References |
- Antineoplastic Agents
- Insulin-Like Growth Factor Binding Protein 3
- RNA, Messenger
- RNA, Small Interfering
- Vitamin E
- Tocopherols
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Topics |
- Animals
- Antineoplastic Agents
(pharmacology)
- Apoptosis
(drug effects)
- Blotting, Western
- Enzyme-Linked Immunosorbent Assay
- Humans
- Immunohistochemistry
- Insulin-Like Growth Factor Binding Protein 3
(drug effects, metabolism)
- Male
- Mice
- Mice, Nude
- Mice, Transgenic
- Prostatic Neoplasms
(drug therapy)
- RNA, Messenger
(analysis)
- RNA, Small Interfering
- Reverse Transcriptase Polymerase Chain Reaction
- Tocopherols
- Vitamin E
(analogs & derivatives, pharmacology)
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